2024
The interplay of δ-subunit and RNA polymerase in the pre-initiation state
ČERNÝ, Martin; Anna KOZÁKOVÁ; Klára MIKESKOVÁ; Hana ŠANDEROVÁ; Karel ŠKUBNÍK et. al.Základní údaje
Originální název
The interplay of δ-subunit and RNA polymerase in the pre-initiation state
Autoři
ČERNÝ, Martin; Anna KOZÁKOVÁ; Klára MIKESKOVÁ; Hana ŠANDEROVÁ; Karel ŠKUBNÍK; Libor KRÁSNÝ a Lukáš ŽÍDEK
Vydání
7th cryoNET Symposium, 2024
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10608 Biochemistry and molecular biology
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Středoevropský technologický institut
Klíčová slova česky
RNA polymeráza, transkripce, iniciace, cryo-EM, RpoE, SigA, SigB
Klíčová slova anglicky
RNA polymerase, transcription, initiation, cryo-EM, RpoE, SigA, SigB
Změněno: 23. 10. 2024 14:18, Mgr. Martin Černý
Anotace
V originále
In prokaryotes, RNA polymerase (RNAP) is a key element in the transcription of genetic information. In order to be efficient and tightly regulated, many microorganisms developed a wide array of transcription factors and auxiliary proteins. In some G+ bacteria, the RNA polymerase is associated with the δ-subunit. The roughly 20 kDa protein consists of 2 domains. The N-terminal which is ordered and facilitates binding to the core, whilst the long negatively charged C-terminal domain is disordered. Previous studies have shown both in in vivo and in vitro studies, that δ-subunit affects the transcription of many genes and its deletion can affect critical processes such as virulence and sporulation. Unfortunately, this step was never structurally characterized. Through the aid of cryoelectron microscopy, we try to uncover the possible interplay between the δ-subunit and the RNAP core, with a focus on the assembly of the pre-initiation complexes, including the σ-factors.
Návaznosti
| GA22-12023S, projekt VaV |
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