SALBUTAMOL ATTENUATES THE ARRHYTHMOGENIC EFFECT OF
AMINOPHYLLINE IN CARDIAC ORGANOIDS EXPERIMENTAL MODEL

a 2024

SALBUTAMOL ATTENUATES THE ARRHYTHMOGENIC EFFECT OF AMINOPHYLLINE IN CARDIAC ORGANOIDS EXPERIMENTAL MODEL

PEŠL, Martin; Daniil KABANOV; Šimon VRANA; Deborah BECKEROVÁ; Martin MOLČAN et. al.

Základní údaje

Originální název

SALBUTAMOL ATTENUATES THE ARRHYTHMOGENIC EFFECT OF AMINOPHYLLINE IN CARDIAC ORGANOIDS EXPERIMENTAL MODEL

Autoři

PEŠL, Martin (203 Česká republika, domácí); Daniil KABANOV (203 Česká republika); Šimon VRANA (203 Česká republika, domácí); Deborah BECKEROVÁ (703 Slovensko, domácí); Martin MOLČAN (203 Česká republika); Martin ŠČUREK (703 Slovensko, domácí); Kristián BRAT (203 Česká republika, domácí); Markéta BÉBAROVÁ (203 Česká republika, domácí); Vladimír ROTREKL (203 Česká republika, domácí); Jan PŘIBYL (203 Česká republika, domácí) a Zdeněk STÁREK (203 Česká republika, domácí)

Vydání

Czech Cardiovascular Research and Innovation Days 2024. 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/24:00137446

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Salbutamol; Aminophylline; Atomic force microscopy; iPSC; HESC; Cardiomyocytes; Pulmonary drug screening; Drug cardiotoxicity; Biomechanical properties; Arrhythmogenic effects

Změněno: 24. 3. 2025 12:08, Mgr. Tereza Miškechová

Anotace

V originále

Background: The combination of aminophylline and salbutamol is used in the treatment of obstructive lung diseases. Side effects (including arrhythmias) of the individual bronchodilator drugs were described, those of combined treatment are almost unknown. We aimed to study the arrhythmogenic potential of combined aminophylline and salbutamol in vitro. Methods: We used the established model of atomic force microscopy (AFM) coupled with cardiac organoids from human pluripotent stem cells (hPSC-CMs). We focused on the chronotropic, inotropic, and arrhythmogenic effects of Salbutamol and Aminophylline alone and combined. Results: Salbutamol and Aminophylline had a synergistic chronotropic and inotropic effects compared to the effects of their separate application. Salbutamol reduced the arrhythmogenic effect of aminophylline, most likely mediated by endothelial nitric oxide synthase activated by beta-2 adrenergic receptors. Findings were replicated and confirmed using hPSC-CM derived from 2 cell lines (CCTL14 and CCTL12). Conclusions: Data suggest that salbutamol as an add-on therapy may not only deliver a bronchodilator effect but also increase the cardiovascular safety of aminophylline, as salbutamol reduces its arrhythmogenic potential. Acknowledgement CarDia (EXCELES, No. LX22NPO5104) EU Next Generation and by the EC Horizon Europe MUQUABIS GA no. 101070546. and MUNI/A/1624/2023 MEYS CR

Návaznosti

LM2023069, projekt VaV

Název: Výzkumná infrastruktura RECETOX

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LX22NPO5104, projekt VaV

Název: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění (Akronym: CarDia)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut pro léčbu metabolických a kardiovaskulárních onemocnění, 5.1 EXCELES

MUNI/A/1624/2023, interní kód MU

Název: Srdeční selhání, jeho komorbidity a etiologické příčiny

Investor: Masarykova univerzita, Srdeční selhání, jeho komorbidity a etiologické příčiny

NU20-06-00156, projekt VaV

Název: Vliv pneumologické medikace na funkce lidských kardiomyocytů.

Investor: Ministerstvo zdravotnictví ČR, Vliv pneumologické medikace na funkce lidských kardiomyocytů, Podprogram 1 - standardní

Citovat

PEŠL, Martin; Daniil KABANOV; Šimon VRANA; Deborah BECKEROVÁ; Martin MOLČAN; Martin ŠČUREK; Kristián BRAT; Markéta BÉBAROVÁ; Vladimír ROTREKL; Jan PŘIBYL a Zdeněk STÁREK. SALBUTAMOL ATTENUATES THE ARRHYTHMOGENIC EFFECT OF AMINOPHYLLINE IN CARDIAC ORGANOIDS EXPERIMENTAL MODEL. In Czech Cardiovascular Research and Innovation Days 2024. 2024.

@proceedings{2446498,
   author = {Pešl, Martin and Kabanov, Daniil and Vrana, Šimon and Beckerová, Deborah and Molčan, Martin and Ščurek, Martin and Brat, Kristián and Bébarová, Markéta and Rotrekl, Vladimír and Přibyl, Jan and Stárek, Zdeněk},
   booktitle = {Czech Cardiovascular Research and Innovation Days 2024.},
   keywords = {Salbutamol; Aminophylline; Atomic force microscopy; iPSC; HESC; Cardiomyocytes; Pulmonary drug screening; Drug cardiotoxicity; Biomechanical properties; Arrhythmogenic effects},
   language = {eng},
   title = {SALBUTAMOL ATTENUATES THE ARRHYTHMOGENIC EFFECT OF AMINOPHYLLINE IN CARDIAC ORGANOIDS EXPERIMENTAL MODEL},
   url = {https://www.cksonline.cz/czech-cardiovascular-research-and-innovation-day-2024/sjezd.php?p=read_abstrakt_program&idabstrakta=70},
   year = {2024}
}

TY  - CONF
ID  - 2446498
AU  - Pešl, Martin - Kabanov, Daniil - Vrana, Šimon - Beckerová, Deborah - Molčan, Martin - Ščurek, Martin - Brat, Kristián - Bébarová, Markéta - Rotrekl, Vladimír - Přibyl, Jan - Stárek, Zdeněk
PY  - 2024
TI  - SALBUTAMOL ATTENUATES THE ARRHYTHMOGENIC EFFECT OF AMINOPHYLLINE IN CARDIAC ORGANOIDS EXPERIMENTAL MODEL
KW  - Salbutamol
KW  - Aminophylline
KW  - Atomic force microscopy
KW  - iPSC
KW  - HESC
KW  - Cardiomyocytes
KW  - Pulmonary drug screening
KW  - Drug cardiotoxicity
KW  - Biomechanical properties
KW  - Arrhythmogenic effects
UR  - https://www.cksonline.cz/czech-cardiovascular-research-and-innovation-day-2024/sjezd.php?p=read_abstrakt_program&idabstrakta=70
N2  - Background: The combination of aminophylline and salbutamol is used in the treatment of obstructive lung diseases. Side effects (including arrhythmias) of the individual bronchodilator drugs were described, those of combined treatment are almost unknown. We aimed to study the arrhythmogenic potential of combined aminophylline and salbutamol in vitro. Methods: We used the established model of atomic force microscopy (AFM) coupled with cardiac organoids from human pluripotent stem cells (hPSC-CMs). We focused on the chronotropic, inotropic, and arrhythmogenic effects of Salbutamol and Aminophylline alone and combined. Results: Salbutamol and Aminophylline had a synergistic chronotropic and inotropic effects compared to the effects of their separate application. Salbutamol reduced the arrhythmogenic effect of aminophylline, most likely mediated by endothelial nitric oxide synthase activated by beta-2 adrenergic receptors. Findings were replicated and confirmed using hPSC-CM derived from 2 cell lines (CCTL14 and CCTL12). Conclusions: Data suggest that salbutamol as an add-on therapy may not only deliver a bronchodilator effect but also increase the cardiovascular safety of aminophylline, as salbutamol reduces its arrhythmogenic potential. Acknowledgement CarDia (EXCELES, No. LX22NPO5104) EU Next Generation and by the EC Horizon Europe MUQUABIS GA no. 101070546. and MUNI/A/1624/2023 MEYS CR
ER  -

PEŠL, Martin; Daniil KABANOV; Šimon VRANA; Deborah BECKEROVÁ; Martin MOLČAN; Martin ŠČUREK; Kristián BRAT; Markéta BÉBAROVÁ; Vladimír ROTREKL; Jan PŘIBYL a Zdeněk STÁREK. SALBUTAMOL ATTENUATES THE ARRHYTHMOGENIC EFFECT OF AMINOPHYLLINE IN CARDIAC ORGANOIDS EXPERIMENTAL MODEL. In \textit{Czech Cardiovascular Research and Innovation Days 2024.}. 2024.

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