The Superior Effect of Radiofrequency With Targeted Ultrasound
for Facial Rejuvenation by Inducing Hyaluronic Acid Synthesis:
A Pilot Preclinical Study

J 2024

The Superior Effect of Radiofrequency With Targeted Ultrasound for Facial Rejuvenation by Inducing Hyaluronic Acid Synthesis: A Pilot Preclinical Study

DUNCAN, Diane; Jan BERNARDY; Nikola HODKOVICOVA; Josef MASEK; Markéta PROCHÁZKOVÁ et. al.

Základní údaje

Originální název

The Superior Effect of Radiofrequency With Targeted Ultrasound for Facial Rejuvenation by Inducing Hyaluronic Acid Synthesis: A Pilot Preclinical Study

Autoři

DUNCAN, Diane; Jan BERNARDY; Nikola HODKOVICOVA; Josef MASEK; Markéta PROCHÁZKOVÁ (203 Česká republika, domácí) a Rea JAROSOVA

Vydání

Aesthetic Surgery Journal Open Forum, Oxford University Press, 2024, 2631-4797

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30212 Surgery

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.600 v roce 2023

Kód RIV

RIV/00216224:14310/24:00138662

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1093/asjof/ojae005

UT WoS

001163645700001

Klíčová slova anglicky

ultrasonography; extracellular matrix; epidermis; fibroblasts; hyaluronic acid; lasers; ligase; matrix-assisted laser desorption-ionization; enzymes; skin biopsies; levels of evidence; quantitative real-time polymerase chain reaction; facial rejuvenation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 1. 2025 08:47, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Background: The level of dermal hyaluronic acid (HA) can be depleted by 75% at age 70. HA provides dermal hydration, volume, and thickness, making it a major component of the extracellular matrix. Restoration of dermal and epidermal HA can be achieved by combining radiofrequency (RF) energy and targeted ultrasound (TUS). The monopolar RF generates heat, with the TUS stimulating HA production. The heat induces a regenerative response in the skin, increasing the fibroblast activity and producing various extracellular matrix compounds, including HA. Objectives: To investigate the effect of the simultaneous application of RF + TUS or RF + US on the stimulation of HA production. Methods: Twelve animals underwent 4 treatments. Six were treated with transcutaneous RF + TUS and 6 with the combination RF + US. The opposite untreated side served as a control. Punch biopsies of the skin were taken at baseline, immediately posttreatment, 1 month, and 2 months posttreatment. The tissue was evaluated with real-time quantitative polymerase chain reaction (RT-qPCR), matrix-assisted laser desorption (MALDI) and time of flight (TOF), and confocal microscopy. Results: The RT-qPCR focused on assessing the production of has1 and has2, enzymes responsible for HA synthesis. RT-qPCR results of the RF + TUS group revealed a +98% and +45% increase in hyaluronic synthetase (HAS) 1 and HAS2 production after the treatments, respectively. The MALDI-TOF revealed a +224% increase in measured HA 2 months after the treatments. The changes were also visible in the confocal microscopy. The control group showed no significant (P > .05) results in either of the evaluation methods. Conclusions: Concurrent application of RF and TUS significantly enhances the natural regenerative processes in skin tissue.

Citovat

DUNCAN, Diane; Jan BERNARDY; Nikola HODKOVICOVA; Josef MASEK; Markéta PROCHÁZKOVÁ a Rea JAROSOVA. The Superior Effect of Radiofrequency With Targeted Ultrasound for Facial Rejuvenation by Inducing Hyaluronic Acid Synthesis: A Pilot Preclinical Study. Aesthetic Surgery Journal Open Forum. Oxford University Press, 2024, roč. 6, January, s. 1-8. ISSN 2631-4797. Dostupné z: https://dx.doi.org/10.1093/asjof/ojae005.

@article{2471874,
   author = {Duncan, Diane and Bernardy, Jan and Hodkovicova, Nikola and Masek, Josef and Procházková, Markéta and Jarosova, Rea},
   article_number = {January},
   doi = {http://dx.doi.org/10.1093/asjof/ojae005},
   keywords = {ultrasonography; extracellular matrix; epidermis; fibroblasts; hyaluronic acid; lasers; ligase; matrix-assisted laser desorption-ionization; enzymes; skin biopsies; levels of evidence; quantitative real-time polymerase chain reaction; facial rejuvenation},
   language = {eng},
   issn = {2631-4797},
   journal = {Aesthetic Surgery Journal Open Forum},
   title = {The Superior Effect of Radiofrequency With Targeted Ultrasound for Facial Rejuvenation by Inducing Hyaluronic Acid Synthesis: A Pilot Preclinical Study},
   url = {https://academic.oup.com/asjopenforum/article/doi/10.1093/asjof/ojae005/7593491},
   volume = {6},
   year = {2024}
}

TY  - JOUR
ID  - 2471874
AU  - Duncan, Diane - Bernardy, Jan - Hodkovicova, Nikola - Masek, Josef - Procházková, Markéta - Jarosova, Rea
PY  - 2024
TI  - The Superior Effect of Radiofrequency With Targeted Ultrasound for Facial Rejuvenation by Inducing Hyaluronic Acid Synthesis: A Pilot Preclinical Study
JF  - Aesthetic Surgery Journal Open Forum
VL  - 6
IS  - January
SP  - 1-8
EP  - 1-8
PB  - Oxford University Press
SN  - 26314797
KW  - ultrasonography
KW  - extracellular matrix
KW  - epidermis
KW  - fibroblasts
KW  - hyaluronic acid
KW  - lasers
KW  - ligase
KW  - matrix-assisted laser desorption-ionization
KW  - enzymes
KW  - skin biopsies
KW  - levels of evidence
KW  - quantitative real-time polymerase chain reaction
KW  - facial rejuvenation
UR  - https://academic.oup.com/asjopenforum/article/doi/10.1093/asjof/ojae005/7593491
N2  - Background: The level of dermal hyaluronic acid (HA) can be depleted by 75% at age 70. HA provides dermal hydration, volume, and thickness, making it a major component of the extracellular matrix. Restoration of dermal and epidermal HA can be achieved by combining radiofrequency (RF) energy and targeted ultrasound (TUS). The monopolar RF generates heat, with the TUS stimulating HA production. The heat induces a regenerative response in the skin, increasing the fibroblast activity and producing various extracellular matrix compounds, including HA. Objectives: To investigate the effect of the simultaneous application of RF + TUS or RF + US on the stimulation of HA production. Methods: Twelve animals underwent 4 treatments. Six were treated with transcutaneous RF + TUS and 6 with the combination RF + US. The opposite untreated side served as a control. Punch biopsies of the skin were taken at baseline, immediately posttreatment, 1 month, and 2 months posttreatment. The tissue was evaluated with real-time quantitative polymerase chain reaction (RT-qPCR), matrix-assisted laser desorption (MALDI) and time of flight (TOF), and confocal microscopy. Results: The RT-qPCR focused on assessing the production of has1 and has2, enzymes responsible for HA synthesis. RT-qPCR results of the RF + TUS group revealed a +98% and +45% increase in hyaluronic synthetase (HAS) 1 and HAS2 production after the treatments, respectively. The MALDI-TOF revealed a +224% increase in measured HA 2 months after the treatments. The changes were also visible in the confocal microscopy. The control group showed no significant (P > .05) results in either of the evaluation methods. Conclusions: Concurrent application of RF and TUS significantly enhances the natural regenerative processes in skin tissue.
ER  -

DUNCAN, Diane; Jan BERNARDY; Nikola HODKOVICOVA; Josef MASEK; Markéta PROCHÁZKOVÁ a Rea JAROSOVA. The Superior Effect of Radiofrequency With Targeted Ultrasound for Facial Rejuvenation by Inducing Hyaluronic Acid Synthesis: A Pilot Preclinical Study. \textit{Aesthetic Surgery Journal Open Forum}. Oxford University Press, 2024, roč.~6, January, s.~1-8. ISSN~2631-4797. Dostupné z: https://dx.doi.org/10.1093/asjof/ojae005.

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