Role of B cells in intratumoral MBTA immunotherapy of murine
pheochromocytoma model

J 2025

Role of B cells in intratumoral MBTA immunotherapy of murine pheochromocytoma model

UHER, Ondrej; Katerina HADRAVA VANOVA; Kateřina PETRLÁKOVÁ; Rachael LABITT; Radka LENCOVA et. al.

Basic information

Original name

Role of B cells in intratumoral MBTA immunotherapy of murine pheochromocytoma model

Authors

UHER, Ondrej (203 Czech Republic); Katerina HADRAVA VANOVA (203 Czech Republic); Kateřina PETRLÁKOVÁ (203 Czech Republic, belonging to the institution); Rachael LABITT; Radka LENCOVA (203 Czech Republic); Andrea FREJLACHOVA (203 Czech Republic); Juan YE; Herui WANG; Michal MASAŘÍK (203 Czech Republic, belonging to the institution); Jan ZENKA (203 Czech Republic); Zhengping ZHUANG and Karel PACAK (203 Czech Republic)

Edition

BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM, London, ELSEVIER SCI LTD, 2025, 1521-690X

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30202 Endocrinology and metabolism

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 6.100 in 2023

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.beem.2024.101941

UT WoS

001420745200001

EID Scopus

2-s2.0-85203831067

Keywords in English

pheochromocytoma; B cells; intratumoral immunotherapy; cytokine storm; melanoma

Abstract

V originále

Immunotherapy represents a revolutionary advancement in cancer treatment, which has traditionally focused on T cells; however, the role of B cells in cancer immunotherapy has gained interest because of their role in antigen presentation, antibody production, and cytokine release. In this study, we examined the role of B cells in previously developed intratumoral MBTA therapy (mannan-BAM, TLR ligands, and anti-CD40 antibody) in murine models of MTT pheochromocytoma. The results indicated that B cells significantly enhance the success of MBTA therapy, with wild-type mice exhibiting a lower tumor incidence and smaller tumors compared with B cell-deficient mice. Increased IL-6 and TNF-alpha levels indicated severe inflammation and a potential cytokine storm in B cell-deficient mice. Neutralization of TNF-alpha ameliorated these complications but resulted in increased tumor recurrence. The results highlight the important role of B cells in enhancing the immune response and maintaining immune homeostasis during MBTA therapy. Our findings offer new insights into improving therapeutic outcomes.

Links

LUAUS24120, research and development project

Name: Synergické cílení klíčových znaků nádorových buněk a jejich mikroprostředí

Investor: Ministry of Education, Youth and Sports of the CR, Synergistic targeting of hallmarks of cancer cells and their microenvironment, INTER-ACTION (USA)

Cite

UHER, Ondrej; Katerina HADRAVA VANOVA; Kateřina PETRLÁKOVÁ; Rachael LABITT; Radka LENCOVA; Andrea FREJLACHOVA; Juan YE; Herui WANG; Michal MASAŘÍK; Jan ZENKA; Zhengping ZHUANG and Karel PACAK. Role of B cells in intratumoral MBTA immunotherapy of murine pheochromocytoma model. BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM. London: ELSEVIER SCI LTD, 2025, vol. 39, No 1, p. 1-10. ISSN 1521-690X. Available from: https://dx.doi.org/10.1016/j.beem.2024.101941.

@article{2481377,
   author = {Uher, Ondrej and Hadrava Vanova, Katerina and Petrláková, Kateřina and Labitt, Rachael and Lencova, Radka and Frejlachova, Andrea and Ye, Juan and Wang, Herui and Masařík, Michal and Zenka, Jan and Zhuang, Zhengping and Pacak, Karel},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.beem.2024.101941},
   keywords = {pheochromocytoma; B cells; intratumoral immunotherapy; cytokine storm; melanoma},
   language = {eng},
   issn = {1521-690X},
   journal = {BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM},
   title = {Role of B cells in intratumoral MBTA immunotherapy of murine pheochromocytoma model},
   url = {https://www.sciencedirect.com/science/article/pii/S1521690X24001179?via%3Dihub},
   volume = {39},
   year = {2025}
}

TY  - JOUR
ID  - 2481377
AU  - Uher, Ondrej - Hadrava Vanova, Katerina - Petrláková, Kateřina - Labitt, Rachael - Lencova, Radka - Frejlachova, Andrea - Ye, Juan - Wang, Herui - Masařík, Michal - Zenka, Jan - Zhuang, Zhengping - Pacak, Karel
PY  - 2025
TI  - Role of B cells in intratumoral MBTA immunotherapy of murine pheochromocytoma model
JF  - BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM
VL  - 39
IS  - 1
SP  - 1-10
EP  - 1-10
PB  - ELSEVIER SCI LTD
SN  - 1521690X
KW  - pheochromocytoma
KW  - B cells
KW  - intratumoral immunotherapy
KW  - cytokine storm
KW  - melanoma
UR  - https://www.sciencedirect.com/science/article/pii/S1521690X24001179?via%3Dihub
N2  - Immunotherapy represents a revolutionary advancement in cancer treatment, which has traditionally focused on T cells; however, the role of B cells in cancer immunotherapy has gained interest because of their role in antigen presentation, antibody production, and cytokine release. In this study, we examined the role of B cells in previously developed intratumoral MBTA therapy (mannan-BAM, TLR ligands, and anti-CD40 antibody) in murine models of MTT pheochromocytoma. The results indicated that B cells significantly enhance the success of MBTA therapy, with wild-type mice exhibiting a lower tumor incidence and smaller tumors compared with B cell-deficient mice. Increased IL-6 and TNF-alpha levels indicated severe inflammation and a potential cytokine storm in B cell-deficient mice. Neutralization of TNF-alpha ameliorated these complications but resulted in increased tumor recurrence. The results highlight the important role of B cells in enhancing the immune response and maintaining immune homeostasis during MBTA therapy. Our findings offer new insights into improving therapeutic outcomes.
ER  -

UHER, Ondrej; Katerina HADRAVA VANOVA; Kateřina PETRLÁKOVÁ; Rachael LABITT; Radka LENCOVA; Andrea FREJLACHOVA; Juan YE; Herui WANG; Michal MASAŘÍK; Jan ZENKA; Zhengping ZHUANG and Karel PACAK. Role of B cells in intratumoral MBTA immunotherapy of murine pheochromocytoma model. \textit{BEST PRACTICE \&{}amp; RESEARCH CLINICAL ENDOCRINOLOGY \&{}amp; METABOLISM}. London: ELSEVIER SCI LTD, 2025, vol.~39, No~1, p.~1-10. ISSN~1521-690X. Available from: https://dx.doi.org/10.1016/j.beem.2024.101941.

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