Advances in engineering substrate scope of Pseudomonas cell
factories

J 2025

Advances in engineering substrate scope of Pseudomonas cell factories

FERREIRA DA SILVA, Miguel Joao, Stefano DONATI and Pavel DVOŘÁK

Basic information

Original name

Advances in engineering substrate scope of Pseudomonas cell factories

Authors

FERREIRA DA SILVA, Miguel Joao (620 Portugal, belonging to the institution), Stefano DONATI and Pavel DVOŘÁK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Current Opinion in Biotechnology, Elsevier Ltd, 2025, 0958-1669

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

20801 Environmental biotechnology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 7.100 in 2023

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.copbio.2025.103270

UT WoS

001429955600001

EID Scopus

2-s2.0-85217921785

Keywords in English

CO-UTILIZATION; D-XYLOSE; PUTIDA STRAINS; METABOLISM; GENOME; WASTE; BIODEGRADATION; CELLOBIOSE; PLASTICS; KT2440

Abstract

V originále

Most current industrial bioprocesses use well-established model microorganisms and simple sugar substrates from edible starch or molasses. To broaden and sustain bioprocesses, we need to explore new robust microbial hosts with desirable traits and ideally exploit diverse waste-derived substrates. Pseudomonas species are prime candidates for new generation of industrial biotechnology due to their resilient physiology and adaptable metabolism. However, natural isolates are not always suitable for demanding biotechnological applications. Despite pseudomonads’ typically broad substrate range, their catabolism can be further enhanced through metabolic engineering, synthetic biology, and/or laboratory evolution to efficiently degrade, utilize, and valorize alternative waste substrates derived from lignocellulosic residues, synthetic plastics, C1 compounds, or their mixtures.

Links

EH22_010/0007541, research and development project

Name: MSCAfellow6_MUNI

GA22-12505S, research and development project

Name: Syntetické konsorcium kmenů Pseudomonas putida pro biodegradaci a ko-utilizaci (hemi)celulózových polymerů

Investor: Czech Science Foundation, Synthetic consortium of Pseudomonas putida strains for biodegradation and co-utilisation of (hemi)cellulosic polymers.

MUNI/J/0003/2021, interní kód MU

Name: A new generation bacterial platform for lignocellulose biotechnology (Acronym: NEWGEN)

Investor: Masaryk University, MASH JUNIOR - MUNI Award In Science and Humanities JUNIOR

Cite

FERREIRA DA SILVA, Miguel Joao, Stefano DONATI and Pavel DVOŘÁK. Advances in engineering substrate scope of Pseudomonas cell factories. Current Opinion in Biotechnology. Elsevier Ltd, 2025, vol. 92, April, p. 1-10. ISSN 0958-1669. Available from: https://dx.doi.org/10.1016/j.copbio.2025.103270.

@article{2481402,
   author = {Ferreira da Silva, Miguel Joao and Donati, Stefano and Dvořák, Pavel},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.copbio.2025.103270},
   keywords = {CO-UTILIZATION; D-XYLOSE; PUTIDA STRAINS; METABOLISM; GENOME; WASTE; BIODEGRADATION; CELLOBIOSE; PLASTICS; KT2440},
   language = {eng},
   issn = {0958-1669},
   journal = {Current Opinion in Biotechnology},
   title = {Advances in engineering substrate scope of Pseudomonas cell factories},
   url = {https://doi.org/10.1016/j.copbio.2025.103270},
   volume = {92},
   year = {2025}
}

TY  - JOUR
ID  - 2481402
AU  - Ferreira da Silva, Miguel Joao - Donati, Stefano - Dvořák, Pavel
PY  - 2025
TI  - Advances in engineering substrate scope of Pseudomonas cell factories
JF  - Current Opinion in Biotechnology
VL  - 92
IS  - April
SP  - 1-10
EP  - 1-10
PB  - Elsevier Ltd
SN  - 09581669
KW  - CO-UTILIZATION
KW  - D-XYLOSE
KW  - PUTIDA STRAINS
KW  - METABOLISM
KW  - GENOME
KW  - WASTE
KW  - BIODEGRADATION
KW  - CELLOBIOSE
KW  - PLASTICS
KW  - KT2440
UR  - https://doi.org/10.1016/j.copbio.2025.103270
N2  - Most current industrial bioprocesses use well-established model microorganisms and simple sugar substrates from edible starch or molasses. To broaden and sustain bioprocesses, we need to explore new robust microbial hosts with desirable traits and ideally exploit diverse waste-derived substrates. Pseudomonas species are prime candidates for new generation of industrial biotechnology due to their resilient physiology and adaptable metabolism. However, natural isolates are not always suitable for demanding biotechnological applications. Despite pseudomonads’ typically broad substrate range, their catabolism can be further enhanced through metabolic engineering, synthetic biology, and/or laboratory evolution to efficiently degrade, utilize, and valorize alternative waste substrates derived from lignocellulosic residues, synthetic plastics, C1 compounds, or their mixtures.
ER  -

FERREIRA DA SILVA, Miguel Joao, Stefano DONATI and Pavel DVOŘÁK. Advances in engineering substrate scope of Pseudomonas cell factories. \textit{Current Opinion in Biotechnology}. Elsevier Ltd, 2025, vol.~92, April, p.~1-10. ISSN~0958-1669. Available from: https://dx.doi.org/10.1016/j.copbio.2025.103270.

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