Assessment of Agrimonia eupatoria L. and Lipophosphonoxin
(DR-6180) Combination for Wound Repair: Bridging the Gap
Between Phytomedicine and Organic Chemistry

J 2024

Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

KAŇUCHOVÁ, Miriam; Veronika BRINDZA LACHOVÁ; Kateřina BOGDANOVÁ; Jana SABOVÁ; Petra BONOVÁ et. al.

Basic information

Original name

Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry

Authors

KAŇUCHOVÁ, Miriam (703 Slovakia, guarantor); Veronika BRINDZA LACHOVÁ (705 Slovenia); Kateřina BOGDANOVÁ (203 Czech Republic); Jana SABOVÁ (703 Slovakia); Petra BONOVÁ (703 Slovakia); Tomáš VASILENKO (703 Slovakia); Ivan KOVÁČ (703 Slovakia); Martin NOVOTNÝ (703 Slovakia); Petra MITRENGOVÁ (703 Slovakia); Nitjawan SAHATSAPAN (703 Slovakia); Matúš COMA (703 Slovakia); Emil ŠVAJDLENKA (203 Czech Republic, belonging to the institution); Milan KOLÁŘ (203 Czech Republic); Peter BOHUŠ (703 Slovakia); Pavel MUČAJI (703 Slovakia); Robert ZAJÍČEK (203 Czech Republic); Dominik REJMAN (203 Czech Republic) and Peter GÁL (703 Slovakia)

Edition

Biomolecules, Basel, Switzerland, MDPI AG, 2024, 2218-273X

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 4.800 in 2023

RIV identification code

RIV/00216224:14160/24:00139010

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.3390/biom14121590

UT WoS

001386946400001

EID Scopus

2-s2.0-85213372135

Keywords in English

skin tissue; extracellular matrix; repair; regeneration; phytotherapy

Abstract

V originále

Agrimonia eupatoria L. (AE) has a rich tradition of use in wound healing improvement across various cultures worldwide. In previous studies, we revealed that Agrimonia eupatoria L. water extract (AE) possesses a rich polyphenolic composition, displaying remarkable antioxidant properties. Our investigations also demonstrated that lipophosphonoxin (LPPO) exhibited antibacterial efficacy in vitro while preserving the proliferation and differentiation of fibroblasts and keratinocytes. Building upon our prior findings, in this study, we intended to examine whether a combination of AE and LPPO could enhance skin wound healing while retaining antibacterial attributes. The antibacterial activity of AE/LPPO against Staphylococcus aureus was evaluated, alongside its effects on fibroblast-to-myofibroblast transition, the formation of extracellular matrix (ECM), and endothelial cells and keratinocyte proliferation/phenotype. We also investigated AE/LPPO's impact on TGF-beta 1 and VEGF-A signaling in keratinocytes/fibroblasts and endothelial cells, respectively. Additionally, wound healing progression in rats was examined through macroscopic observation and histological analysis. Our results indicate that AE/LPPO promotes myofibroblast-like phenotypic changes and augments ECM deposition. Clinically relevant, the AE/LPPO did not disrupt TGF-beta 1 and VEGF-A signaling and accelerated wound closure in rats. Notably, while AE and LPPO individually exhibited antibacterial activity, their combination did not lead to synergism, rather decreasing antibacterial activity, warranting further examination. These findings underscore substantial wound healing improvement facilitated by AE/LPPO, requiring further exploration in animal models closer to human physiology.

Links

LX22NPO5103, research and development project

Name: Národní institut virologie a bakteriologie (Acronym: NIVB)

Investor: Ministry of Education, Youth and Sports of the CR, National Institute of Virology and Bacteriology, 5.1 EXCELES

Cite

KAŇUCHOVÁ, Miriam; Veronika BRINDZA LACHOVÁ; Kateřina BOGDANOVÁ; Jana SABOVÁ; Petra BONOVÁ; Tomáš VASILENKO; Ivan KOVÁČ; Martin NOVOTNÝ; Petra MITRENGOVÁ; Nitjawan SAHATSAPAN; Matúš COMA; Emil ŠVAJDLENKA; Milan KOLÁŘ; Peter BOHUŠ; Pavel MUČAJI; Robert ZAJÍČEK; Dominik REJMAN and Peter GÁL. Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry. Biomolecules. Basel, Switzerland: MDPI AG, 2024, vol. 14, No 12, p. 1-21. ISSN 2218-273X. Available from: https://dx.doi.org/10.3390/biom14121590.

@article{2481981,
   author = {Kaňuchová, Miriam and Brindza Lachová, Veronika and Bogdanová, Kateřina and Sabová, Jana and Bonová, Petra and Vasilenko, Tomáš and Kováč, Ivan and Novotný, Martin and Mitrengová, Petra and Sahatsapan, Nitjawan and Coma, Matúš and Švajdlenka, Emil and Kolář, Milan and Bohuš, Peter and Mučaji, Pavel and Zajíček, Robert and Rejman, Dominik and Gál, Peter},
   article_location = {Basel, Switzerland},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/biom14121590},
   keywords = {skin tissue; extracellular matrix; repair; regeneration; phytotherapy},
   language = {eng},
   issn = {2218-273X},
   journal = {Biomolecules},
   title = {Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry},
   url = {https://www.mdpi.com/2218-273X/14/12/1590},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2481981
AU  - Kaňuchová, Miriam - Brindza Lachová, Veronika - Bogdanová, Kateřina - Sabová, Jana - Bonová, Petra - Vasilenko, Tomáš - Kováč, Ivan - Novotný, Martin - Mitrengová, Petra - Sahatsapan, Nitjawan - Coma, Matúš - Švajdlenka, Emil - Kolář, Milan - Bohuš, Peter - Mučaji, Pavel - Zajíček, Robert - Rejman, Dominik - Gál, Peter
PY  - 2024
TI  - Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry
JF  - Biomolecules
VL  - 14
IS  - 12
SP  - 1-21
EP  - 1-21
PB  - MDPI AG
SN  - 2218273X
KW  - skin tissue
KW  - extracellular matrix
KW  - repair
KW  - regeneration
KW  - phytotherapy
UR  - https://www.mdpi.com/2218-273X/14/12/1590
N2  - Agrimonia eupatoria L. (AE) has a rich tradition of use in wound healing improvement across various cultures worldwide. In previous studies, we revealed that Agrimonia eupatoria L. water extract (AE) possesses a rich polyphenolic composition, displaying remarkable antioxidant properties. Our investigations also demonstrated that lipophosphonoxin (LPPO) exhibited antibacterial efficacy in vitro while preserving the proliferation and differentiation of fibroblasts and keratinocytes. Building upon our prior findings, in this study, we intended to examine whether a combination of AE and LPPO could enhance skin wound healing while retaining antibacterial attributes. The antibacterial activity of AE/LPPO against Staphylococcus aureus was evaluated, alongside its effects on fibroblast-to-myofibroblast transition, the formation of extracellular matrix (ECM), and endothelial cells and keratinocyte proliferation/phenotype. We also investigated AE/LPPO's impact on TGF-beta 1 and VEGF-A signaling in keratinocytes/fibroblasts and endothelial cells, respectively. Additionally, wound healing progression in rats was examined through macroscopic observation and histological analysis. Our results indicate that AE/LPPO promotes myofibroblast-like phenotypic changes and augments ECM deposition. Clinically relevant, the AE/LPPO did not disrupt TGF-beta 1 and VEGF-A signaling and accelerated wound closure in rats. Notably, while AE and LPPO individually exhibited antibacterial activity, their combination did not lead to synergism, rather decreasing antibacterial activity, warranting further examination. These findings underscore substantial wound healing improvement facilitated by AE/LPPO, requiring further exploration in animal models closer to human physiology.
ER  -

KAŇUCHOVÁ, Miriam; Veronika BRINDZA LACHOVÁ; Kateřina BOGDANOVÁ; Jana SABOVÁ; Petra BONOVÁ; Tomáš VASILENKO; Ivan KOVÁČ; Martin NOVOTNÝ; Petra MITRENGOVÁ; Nitjawan SAHATSAPAN; Matúš COMA; Emil ŠVAJDLENKA; Milan KOLÁŘ; Peter BOHUŠ; Pavel MUČAJI; Robert ZAJÍČEK; Dominik REJMAN and Peter GÁL. Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry. \textit{Biomolecules}. Basel, Switzerland: MDPI AG, 2024, vol.~14, No~12, p.~1-21. ISSN~2218-273X. Available from: https://dx.doi.org/10.3390/biom14121590.

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