Mesenchymal Meis2 controls whisker development independently
from trigeminal sensory innervation

J 2025

Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation

KAPLAN, Mehmet Mahsum; Erika HUDACOVA; Miroslav MATEJCEK; Haneen Riadh Ali TUAIMA; Jan KŘIVÁNEK et. al.

Basic information

Original name

Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation

Authors

KAPLAN, Mehmet Mahsum; Erika HUDACOVA (203 Czech Republic); Miroslav MATEJCEK (203 Czech Republic); Haneen Riadh Ali TUAIMA (276 Germany, belonging to the institution); Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution) and Ondrej MACHON (203 Czech Republic)

Edition

eLife, CAMBRIDGE, eLIFE SCIENCES PUBL LTD, 2025, 2050-084X

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 6.400 in 2023

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.7554/eLife.100854.3

UT WoS

001460019100001

EID Scopus

999

Keywords in English

Meis2; whisker development; mesenchyme; trigeminal nerve; placode formation

Abstract

In the original language

Hair follicle development is initiated by reciprocal molecular interactions between the placode-forming epithelium and the underlying mesenchyme. Cell fate transformation in dermal fibroblasts generates a cell niche for placode induction by activation of signaling pathways WNT, EDA, and FGF in the epithelium. These successive paracrine epithelial signals initiate dermal condensation in the underlying mesenchyme. Although epithelial signaling from the placode to mesenchyme is better described, little is known about primary mesenchymal signals resulting in placode induction. Here we show that Meis2 expression in cells derived from the neural crest is critical for whisker formation, and also for branching of trigeminal nerves. While whisker formation is independent of the trigeminal sensory innervation, MEIS2 in mesenchymal dermal cells orchestrates the initial steps of epithelial placode formation and subsequent dermal condensation. MEIS2 regulates the expression of transcription factor Foxd1, which is typical of pre-dermal condensation. However, deletion of Foxd1 does not affect whisker development. Overall, our data suggest an early role of mesenchymal MEIS2 during whisker formation and provide evidence that whiskers can normally develop in the absence of sensory innervation or Foxd1 expression.

Links

GA23-06160S, research and development project

Name: Fluktuace mikroprostředí kmenových buněk jako zdroj tkáňové adaptability ve zdraví a nemoci

Investor: Czech Science Foundation, Fluctuation of the stem cell niche as a source of tissue adaptability in health and disease

LM2023050, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR, Czech BioImaging: National research infrastructure for biological and medical imaging

MUNI/A/1598/2023, interní kód MU

Name: Zdroje pro tkáňové inženýrství 14

Investor: Masaryk University, Resources pro tissue engineering 14

90124, large research infrastructures

Name: NanoEnviCz II

Cite

KAPLAN, Mehmet Mahsum; Erika HUDACOVA; Miroslav MATEJCEK; Haneen Riadh Ali TUAIMA; Jan KŘIVÁNEK and Ondrej MACHON. Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation. eLife. CAMBRIDGE: eLIFE SCIENCES PUBL LTD, 2025, vol. 13, No 1, p. 1-21. ISSN 2050-084X. Available from: https://doi.org/10.7554/eLife.100854.3.

@article{2488579,
   author = {Kaplan, Mehmet Mahsum and Hudacova, Erika and Matejcek, Miroslav and Tuaima, Haneen Riadh Ali and Křivánek, Jan and Machon, Ondrej},
   article_location = {CAMBRIDGE},
   article_number = {1},
   doi = {https://doi.org/10.7554/eLife.100854.3},
   keywords = {Meis2; whisker development; mesenchyme; trigeminal nerve; placode formation},
   language = {eng},
   issn = {2050-084X},
   journal = {eLife},
   title = {Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation},
   url = {https://elifesciences.org/reviewed-preprints/100854v2#d1e1837},
   volume = {13},
   year = {2025}
}

TY  - JOUR
ID  - 2488579
AU  - Kaplan, Mehmet Mahsum - Hudacova, Erika - Matejcek, Miroslav - Tuaima, Haneen Riadh Ali - Křivánek, Jan - Machon, Ondrej
PY  - 2025
TI  - Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation
JF  - eLife
VL  - 13
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - eLIFE SCIENCES PUBL LTD
SN  - 2050084X
KW  - Meis2
KW  - whisker development
KW  - mesenchyme
KW  - trigeminal nerve
KW  - placode formation
UR  - https://elifesciences.org/reviewed-preprints/100854v2#d1e1837
N2  - Hair follicle development is initiated by reciprocal molecular interactions between the placode-forming epithelium and the underlying mesenchyme. Cell fate transformation in dermal fibroblasts generates a cell niche for placode induction by activation of signaling pathways WNT, EDA, and FGF in the epithelium. These successive paracrine epithelial signals initiate dermal condensation in the underlying mesenchyme. Although epithelial signaling from the placode to mesenchyme is better described, little is known about primary mesenchymal signals resulting in placode induction. Here we show that Meis2 expression in cells derived from the neural crest is critical for whisker formation, and also for branching of trigeminal nerves. While whisker formation is independent of the trigeminal sensory innervation, MEIS2 in mesenchymal dermal cells orchestrates the initial steps of epithelial placode formation and subsequent dermal condensation. MEIS2 regulates the expression of transcription factor Foxd1, which is typical of pre-dermal condensation. However, deletion of Foxd1 does not affect whisker development. Overall, our data suggest an early role of mesenchymal MEIS2 during whisker formation and provide evidence that whiskers can normally develop in the absence of sensory innervation or Foxd1 expression.
ER  -

KAPLAN, Mehmet Mahsum; Erika HUDACOVA; Miroslav MATEJCEK; Haneen Riadh Ali TUAIMA; Jan KŘIVÁNEK and Ondrej MACHON. Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation. \textit{eLife}. CAMBRIDGE: eLIFE SCIENCES PUBL LTD, 2025, vol.~13, No~1, p.~1-21. ISSN~2050-084X. Available from: https://doi.org/10.7554/eLife.100854.3.

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