Organic films with amino groups on nanofibrous structure
control innate immune response

J 2025

Organic films with amino groups on nanofibrous structure control innate immune response

JANŮŠOVÁ, Martina; Patrik MATUŠŮ; Jana BARTOŠÍKOVÁ; Lucie JANŮ; Zdeňka ŠILLEROVÁ et. al.

Základní údaje

Originální název

Organic films with amino groups on nanofibrous structure control innate immune response

Autoři

JANŮŠOVÁ, Martina; Patrik MATUŠŮ; Jana BARTOŠÍKOVÁ; Lucie JANŮ; Zdeňka ŠILLEROVÁ; David NEČAS; Petr RYŠÁNEK; Jiřina MEDALOVÁ a Lenka ZAJÍČKOVÁ

Vydání

Applied Materials Today, Elsevier Ltd. 2025, 2352-9407

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10305 Fluids and plasma physics

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.900 v roce 2024

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.apmt.2025.102965

UT WoS

001604550100001

EID Scopus

2-s2.0-105020566954

Klíčová slova anglicky

Amino-functionalized surfaces; Polymer nanofibers; Immune response; Neutrophils; Macrophages

Štítky

14312020, 14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 11. 2025 14:56, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Synthetic polymer nanofibers, such as polycaprolactone (PCL), are widely used as scaffolds in tissue engineering due to their ease of fabrication. However, their surface properties often fail to meet the specific requirements of cell culture. Coating the scaffolds with organic thin films containing amino groups using a plasma-based dry method provides a simple, single-step, and tunable approach to enhance their hydrophilicity. Motivated by the potential application of these films in bioengineering, this study investigates key characteristics influencing the immune response, capturing both the early and late stages of immune activation represented here by neutrophil-like and macrophage-like cell models, respectively. In neutrophils, the strongest undesirable pro-inflammatory activation was triggered by films with high nitrogen content, positive surface charge, and low water stability, as evidenced by elevated expression of pro-inflammatory cytokines. Macrophage-like cells exhibited a similar trend, albeit with a shifted activation threshold: amine films characterized by lower nitrogen content more effectively reduced pro-inflammatory activation. Morphological changes in macrophage-like cells further supported the role of surface chemistry in modulating their behavior. In addition to surface chemistry, substrate morphology played a role in immune modulation. The porous structure of PCL nanofibers enhanced the immune profile of macrophage-like cells by increasing pro-regenerative M2 cytokine expression and reducing pro-inflammatory M1 markers. In contrast, neutrophil-like cells were largely unaffected by substrate morphology and responded primarily to surface chemistry. This study underscores the importance of immune response investigation in biomaterial design.

Návaznosti

90251, velká výzkumná infrastruktura

Název: CzechNanoLab II

90266, velká výzkumná infrastruktura

Název: NanoEnviCz III

Citovat

JANŮŠOVÁ, Martina; Patrik MATUŠŮ; Jana BARTOŠÍKOVÁ; Lucie JANŮ; Zdeňka ŠILLEROVÁ; David NEČAS; Petr RYŠÁNEK; Jiřina MEDALOVÁ a Lenka ZAJÍČKOVÁ. Organic films with amino groups on nanofibrous structure control innate immune response. Applied Materials Today. Elsevier Ltd., 2025, roč. 47, December, s. 102965-102980. ISSN 2352-9407. Dostupné z: https://doi.org/10.1016/j.apmt.2025.102965.

@article{2530801,
   author = {Janůšová, Martina and Matušů, Patrik and Bartošíková, Jana and Janů, Lucie and Šillerová, Zdeňka and Nečas, David and Ryšánek, Petr and Medalová, Jiřina and Zajíčková, Lenka},
   article_number = {December},
   doi = {https://doi.org/10.1016/j.apmt.2025.102965},
   keywords = {Amino-functionalized surfaces; Polymer nanofibers; Immune response; Neutrophils; Macrophages},
   language = {eng},
   issn = {2352-9407},
   journal = {Applied Materials Today},
   title = {Organic films with amino groups on nanofibrous structure control innate immune response},
   url = {https://doi.org/10.1016/j.apmt.2025.102965},
   volume = {47},
   year = {2025}
}

TY  - JOUR
ID  - 2530801
AU  - Janůšová, Martina - Matušů, Patrik - Bartošíková, Jana - Janů, Lucie - Šillerová, Zdeňka - Nečas, David - Ryšánek, Petr - Medalová, Jiřina - Zajíčková, Lenka
PY  - 2025
TI  - Organic films with amino groups on nanofibrous structure control innate immune response
JF  - Applied Materials Today
VL  - 47
IS  - December
SP  - 102965
EP  - 102965
PB  - Elsevier Ltd.
SN  - 23529407
KW  - Amino-functionalized surfaces
KW  - Polymer nanofibers
KW  - Immune response
KW  - Neutrophils
KW  - Macrophages
UR  - https://doi.org/10.1016/j.apmt.2025.102965
N2  - Synthetic polymer nanofibers, such as polycaprolactone (PCL), are widely used as scaffolds in tissue engineering due to their ease of fabrication. However, their surface properties often fail to meet the specific requirements of cell culture. Coating the scaffolds with organic thin films containing amino groups using a plasma-based dry method provides a simple, single-step, and tunable approach to enhance their hydrophilicity. Motivated by the potential application of these films in bioengineering, this study investigates key characteristics influencing the immune response, capturing both the early and late stages of immune activation represented here by neutrophil-like and macrophage-like cell models, respectively. In neutrophils, the strongest undesirable pro-inflammatory activation was triggered by films with high nitrogen content, positive surface charge, and low water stability, as evidenced by elevated expression of pro-inflammatory cytokines. Macrophage-like cells exhibited a similar trend, albeit with a shifted activation threshold: amine films characterized by lower nitrogen content more effectively reduced pro-inflammatory activation. Morphological changes in macrophage-like cells further supported the role of surface chemistry in modulating their behavior. In addition to surface chemistry, substrate morphology played a role in immune modulation. The porous structure of PCL nanofibers enhanced the immune profile of macrophage-like cells by increasing pro-regenerative M2 cytokine expression and reducing pro-inflammatory M1 markers. In contrast, neutrophil-like cells were largely unaffected by substrate morphology and responded primarily to surface chemistry. This study underscores the importance of immune response investigation in biomaterial design.
ER  -

JANŮŠOVÁ, Martina; Patrik MATUŠŮ; Jana BARTOŠÍKOVÁ; Lucie JANŮ; Zdeňka ŠILLEROVÁ; David NEČAS; Petr RYŠÁNEK; Jiřina MEDALOVÁ a Lenka ZAJÍČKOVÁ. Organic films with amino groups on nanofibrous structure control innate immune response. \textit{Applied Materials Today}. Elsevier Ltd., 2025, roč.~47, December, s.~102965-102980. ISSN~2352-9407. Dostupné z: https://doi.org/10.1016/j.apmt.2025.102965.

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