Nuclear topography of the c-myc gene in human leukemic cells

BÁRTOVÁ, Eva, Stanislav KOZUBEK, Michal KOZUBEK, Pavla JIRSOVÁ, Emilie LUKÁŠOVÁ, Magdalena SKALNÍKOVÁ, Alena CAFOURKOVÁ and Irena KOUTNÁ. Nuclear topography of the c-myc gene in human leukemic cells. Gene. Amsterdam: Elsevier, 2000, vol. 244, No 1, p. 1-11. ISSN 0378-1119.

Basic information

Original name

Nuclear topography of the c-myc gene in human leukemic cells

Authors

BÁRTOVÁ, Eva (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Magdalena SKALNÍKOVÁ (203 Czech Republic), Alena CAFOURKOVÁ (203 Czech Republic) and Irena KOUTNÁ (203 Czech Republic)

Edition

Gene, Amsterdam, Elsevier, 2000, 0378-1119

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

abstract

Impact factor

Impact factor: 2.461

RIV identification code

RIV/00216224:14330/00:00001989

Organization unit

Faculty of Informatics

UT WoS

000085660700001

Keywords in English

c-myc topography; differentiation; FISH; HL-60; U-937

Tags

c-myc topography, differentiation, FISH, HL-60, U-937

Tags

International impact, Reviewed

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Abstract

V originále

myc gene plays an essential role in the regulation of the cell cycle and differentiation. Therefore, changes of the c-myc positioning during differentiation are of great interest. As a model system of cell differentiation, the HL-60 and U-937 human leukemic cell lines, were used in our experiments. These cells can be induced to differentiation into granulocytes that represent one of the pathways of blood cell maturation. In this study, changes of the topographic characteristics of the c-myc gene (8q24), centromeric region of chromosome 8 and chromosome 8 domain during differentiation of HL-60 and U-937 cells were detected using fluorescence in situ hybridisation (FISH). FISH techniques and fluorescence microscopy combined with image acquisition and analysis (High Resolution Cytometry) were used in order to detect the topographic features of nuclear chromatin.

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Links

GA202/97/0874, research and development project	Name: Struktura interfázního jádra a její změny po ozáření Investor: Czech Science Foundation, The structure of interphase nucleus and its changes after irradiation
GA202/98/P253, research and development project	Name: Změna struktury interfázního jádra buněk lidských leukemických linií po působení diferenciačních činidel a záření Investor: Czech Science Foundation, Changes in the structure of interphase nucleai of human leukemic cell lines after treatment with differentiating agents and gama irradiation
MSM 143300002, plan (intention)	Name: Využití počítačové analýzy obrazu v optické mikroskopii Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
NM15, research and development project	Name: Užití interfázní FISH techniky pro vyhledávání lidí se zvýšeným rizikem vzniku zhoubných hemoblastóz Investor: Ministry of Health of the CR, The use interphase FISH for the determination of individuals with elevated risk of the induction of hemoblastoses
VS97031, research and development project	Name: Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka Investor: Ministry of Education, Youth and Sports of the CR, Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases