Nuclear topography of the c-myc gene in human leukemic cells

BÁRTOVÁ, Eva, Stanislav KOZUBEK, Michal KOZUBEK, Pavla JIRSOVÁ, Emilie LUKÁŠOVÁ, Magdalena SKALNÍKOVÁ, Alena CAFOURKOVÁ and Irena KOUTNÁ. Nuclear topography of the c-myc gene in human leukemic cells. Gene. Amsterdam: Elsevier, 2000, vol. 244, No 1, p. 1-11. ISSN 0378-1119.

Basic information

• Original name: Nuclear topography of the c-myc gene in human leukemic cells
• Authors: BÁRTOVÁ, Eva (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Magdalena SKALNÍKOVÁ (203 Czech Republic), Alena CAFOURKOVÁ (203 Czech Republic) and Irena KOUTNÁ (203 Czech Republic).
• Edition: Gene, Amsterdam, Elsevier, 2000, 0378-1119.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: abstract
• Impact factor: Impact factor: 2.461
• RIV identification code: RIV/00216224:14330/00:00001989
• Organization unit: Faculty of Informatics
• UT WoS: 000085660700001
• Keywords in English: c-myc topography; differentiation; FISH; HL-60; U-937
• Tags: c-myc topography, differentiation, FISH, HL-60, U-937
• Tags: International impact, Reviewed

Abstract

myc gene plays an essential role in the regulation of the cell cycle and differentiation. Therefore, changes of the c-myc positioning during differentiation are of great interest. As a model system of cell differentiation, the HL-60 and U-937 human leukemic cell lines, were used in our experiments. These cells can be induced to differentiation into granulocytes that represent one of the pathways of blood cell maturation. In this study, changes of the topographic characteristics of the c-myc gene (8q24), centromeric region of chromosome 8 and chromosome 8 domain during differentiation of HL-60 and U-937 cells were detected using fluorescence in situ hybridisation (FISH). FISH techniques and fluorescence microscopy combined with image acquisition and analysis (High Resolution Cytometry) were used in order to detect the topographic features of nuclear chromatin.

Links

• GA202/97/0874, research and development project: Name: Struktura interfázního jádra a její změny po ozáření

Investor: Czech Science Foundation, The structure of interphase nucleus and its changes after irradiation

• GA202/98/P253, research and development project: Name: Změna struktury interfázního jádra buněk lidských leukemických linií po působení diferenciačních činidel a záření

Investor: Czech Science Foundation, Changes in the structure of interphase nucleai of human leukemic cell lines after treatment with differentiating agents and gama irradiation

• MSM 143300002, plan (intention): Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

• NM15, research and development project: Name: Užití interfázní FISH techniky pro vyhledávání lidí se zvýšeným rizikem vzniku zhoubných hemoblastóz

Investor: Ministry of Health of the CR, The use interphase FISH for the determination of individuals with elevated risk of the induction of hemoblastoses

• VS97031, research and development project: Name: Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka

Investor: Ministry of Education, Youth and Sports of the CR, Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases