J 2000

Application of Elimination Voltammetry to Adsorptive Stripping of DNA

TRNKOVÁ, Libuše, René KIZEK and Oldřich DRAČKA

Basic information

Original name

Application of Elimination Voltammetry to Adsorptive Stripping of DNA

Authors

TRNKOVÁ, Libuše, René KIZEK and Oldřich DRAČKA

Edition

Electroanalysis, Weinheim, Wiley-VCH Verlag GmbH, 2000, 1040-0397

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.972

RIV identification code

RIV/00216224:14310/00:00003066

Organization unit

Faculty of Science

UT WoS

000088963200006

Keywords in English

Linear scan voltametry; Elimination voltammetry; Irreversible current; Adsorptive Stripping Voltammetry; DNA

Tags

Změněno: 12/3/2001 16:45, prof. RNDr. Libuše Trnková, CSc.

Abstract

V originále

Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak (due to the reduction of adenine and cytosine residues), the elimination provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter.

Links

GV204/97/K084, research and development project
Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu
Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research
MSM 143100011, plan (intention)
Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů
Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles