Characterization of oxacillin-resistant coagulase-negative
staphylococcal strains isolated in the Czech Republic

a 2000

Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic

PANTŮČEK, Roman, Václav HÁJEK, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC et. al.

Basic information

Original name

Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic

Authors

PANTŮČEK, Roman (203 Czech Republic), Václav HÁJEK, Ivo SEDLÁČEK (203 Czech Republic), Petr PETRÁŠ, Pavel ŠVEC (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic), Stanislav ROSYPAL (203 Czech Republic), Jana KAILEROVÁ (203 Czech Republic), Jitka FAITOVÁ (203 Czech Republic), Eva ZAHRADNÍČKOVÁ (203 Czech Republic), Roman RYBÁŘ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

9th International Symposium on Staphylococci and Staphylococcal Infections, 2000

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Denmark

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/00:00002296

Organization unit

Faculty of Science

Keywords in English

coagulase negative staphylococci

Změněno: 31/3/2010 11:59, prof. RNDr. Ivo Sedláček, CSc.

Abstract

V originále

The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. The phage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relationships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis indicated the differences in the restriction patterns of all the strains exhibiting similarity 60-95%. On the other hand the ribotyping with the probe 16S-23S rDNA from E. coli led to the estimation of 4 distinct ribotypes only. The PFGE-heterogeneity of a lower degree (similarity 70-100%) was found in the strains S. haemolyticus and S. hominis, which correlates well with the estimated ribotypes. Almost each of the S. haemolyticus strains exhibited a characteristic plasmid profile corresponding to the differences observed in the antibiotics resistance patterns. Seven or 8 different plasmid profiles were found to occur in S. epidermidis and S. hominis strains, respectively. It can be concluded that the strains of all three species under study are characteristic of a considerable genomic variability. For their differentation the PFGE proved to have the highest discriminatory power. Plasmid analysis appears to be suitable as typing method for S. haemolyticus.

Links

GA301/99/D075, research and development project

Name: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

Investor: Czech Science Foundation, Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

PANTŮČEK, Roman, Václav HÁJEK, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC, Vladislava RŮŽIČKOVÁ, Stanislav ROSYPAL, Jana KAILEROVÁ, Jitka FAITOVÁ, Eva ZAHRADNÍČKOVÁ, Roman RYBÁŘ and Jiří DOŠKAŘ. Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic. In 9th International Symposium on Staphylococci and Staphylococcal Infections. 2000.

@proceedings{327191,
   author = {Pantůček, Roman and Hájek, Václav and Sedláček, Ivo and Petráš, Petr and Švec, Pavel and Růžičková, Vladislava and Rosypal, Stanislav and Kailerová, Jana and Faitová, Jitka and Zahradníčková, Eva and Rybář, Roman and Doškař, Jiří},
   booktitle = {9th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {coagulase negative staphylococci},
   language = {eng},
   title = {Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic},
   year = {2000}
}

TY  - CONF
ID  - 327191
AU  - Pantůček, Roman - Hájek, Václav - Sedláček, Ivo - Petráš, Petr - Švec, Pavel - Růžičková, Vladislava - Rosypal, Stanislav - Kailerová, Jana - Faitová, Jitka - Zahradníčková, Eva - Rybář, Roman - Doškař, Jiří
PY  - 2000
TI  - Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic
KW  - coagulase negative staphylococci
N2  - The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. The phage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relationships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis indicated the differences in the restriction patterns of all the strains exhibiting similarity 60-95%. On the other hand the ribotyping with the probe 16S-23S rDNA from E. coli led to the estimation of 4 distinct ribotypes only. The PFGE-heterogeneity of a lower degree (similarity 70-100%) was found in the strains S. haemolyticus and S. hominis, which correlates well with the estimated ribotypes. Almost each of the S. haemolyticus strains exhibited a characteristic plasmid profile corresponding to the differences observed in the antibiotics resistance patterns. Seven or 8 different plasmid profiles were found to occur in S. epidermidis and S. hominis strains, respectively. It can be concluded that the strains of all three species under study are characteristic of a considerable genomic variability. For their differentation the PFGE proved to have the highest discriminatory power. Plasmid analysis appears to be suitable as typing method for S. haemolyticus.
ER  -

PANTŮČEK, Roman, Václav HÁJEK, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC, Vladislava RŮŽIČKOVÁ, Stanislav ROSYPAL, Jana KAILEROVÁ, Jitka FAITOVÁ, Eva ZAHRADNÍČKOVÁ, Roman RYBÁŘ and Jiří DOŠKAŘ. Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic. In \textit{9th International Symposium on Staphylococci and Staphylococcal Infections}. 2000.

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