Detailed Information on Publication Record
2000
Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide
GLATZ, Zdeněk and Hana MAŠLAŇOVÁBasic information
Original name
Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide
Authors
GLATZ, Zdeněk and Hana MAŠLAŇOVÁ
Edition
Journal of Chromatography A, Netherlands, Elsevier, 2000, 0021-9606
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.301
RIV identification code
RIV/00216224:14310/00:00003314
Organization unit
Faculty of Science
Keywords in English
thiol; on-column derivatization; MEKC
Tags
Změněno: 27/2/2001 15:58, prof. RNDr. Zdeněk Glatz, CSc.
Abstract
V originále
A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination.
Links
| GA525/00/0785, research and development project |
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