| GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide. Journal of Chromatography A. Netherlands: Elsevier, 2000, vol. 895, 1-2, p. 179-187. ISSN 0021-9606. |
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@article{329154,
author = {Glatz, Zdeněk and Mašlaňová, Hana},
article_location = {Netherlands},
article_number = {1-2},
keywords = {thiol; on-column derivatization; MEKC},
language = {eng},
issn = {0021-9606},
journal = {Journal of Chromatography A},
title = {Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide},
volume = {895},
year = {2000}
}
TY - JOUR
ID - 329154
AU - Glatz, Zdeněk - Mašlaňová, Hana
PY - 2000
TI - Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide
JF - Journal of Chromatography A
VL - 895
IS - 1-2
SP - 179
EP - 179
PB - Elsevier
SN - 00219606
KW - thiol
KW - on-column derivatization
KW - MEKC
N2 - A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination.
ER -
GLATZ, Zdeněk and Hana MAŠLAŇOVÁ. Specific thiol determination by micellar electrokinetic chromatography and on-column detection reaction with 2,2-dipyridyldisulphide. \textit{Journal of Chromatography A}. Netherlands: Elsevier, 2000, vol.~895, 1-2, p.~179-187. ISSN~0021-9606.
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