Application of Elimination Methods in Electrochemical Study of
DNA

J 2000

Application of Elimination Methods in Electrochemical Study of DNA

TRNKOVÁ, Libuše, René KIZEK and Oldřich DRAČKA

Basic information

Original name

Application of Elimination Methods in Electrochemical Study of DNA

Authors

TRNKOVÁ, Libuše, René KIZEK and Oldřich DRAČKA

Edition

Journal of Biomolecular Structure and Dynamics, GB, A Bimonthly Publication of Adenine Press, 2000, 0739-1102

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.826

RIV identification code

RIV/00216224:14310/00:00003319

Organization unit

Faculty of Science

Keywords in English

Elimination of particular currents; elimination polarography/voltammetry; DNA; adsorbed species

Abstract

V originále

Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak, the EVLS provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter.

Links

GV204/97/K084, research and development project

Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

Citovat

TRNKOVÁ, Libuše, René KIZEK and Oldřich DRAČKA. Application of Elimination Methods in Electrochemical Study of DNA. Journal of Biomolecular Structure and Dynamics. GB: A Bimonthly Publication of Adenine Press, 2000, vol. 17, No 6, p. 1169. ISSN 0739-1102.

@article{330391,
   author = {Trnková, Libuše and Kizek, René and Dračka, Oldřich},
   article_location = {GB},
   article_number = {6},
   keywords = {Elimination of particular currents; elimination polarography/voltammetry; DNA; adsorbed species},
   language = {eng},
   issn = {0739-1102},
   journal = {Journal of Biomolecular Structure and Dynamics},
   title = {Application of Elimination Methods in Electrochemical Study of DNA},
   volume = {17},
   year = {2000}
}

TY  - JOUR
ID  - 330391
AU  - Trnková, Libuše - Kizek, René - Dračka, Oldřich
PY  - 2000
TI  - Application of Elimination Methods in Electrochemical Study of DNA
JF  - Journal of Biomolecular Structure and Dynamics
VL  - 17
IS  - 6
SP  - 1169
EP  - 1169
PB  - A Bimonthly Publication of Adenine Press
SN  - 07391102
KW  - Elimination of particular currents
KW  - elimination polarography/voltammetry
KW  - DNA
KW  - adsorbed species
N2  - Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak, the EVLS provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter.
ER  -

TRNKOVÁ, Libuše, René KIZEK and Oldřich DRAČKA. Application of Elimination Methods in Electrochemical Study of DNA. \textit{Journal of Biomolecular Structure and Dynamics}. GB: A Bimonthly Publication of Adenine Press, 2000, vol.~17, No~6, p.~1169. ISSN~0739-1102.

Detailed Information on Publication Record