ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA and Pavel MUSIL. Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis. In Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000. Bratislava: Slovenská chemická společnost, 2000, p. 85.
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Basic information
Original name Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis
Authors ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA and Pavel MUSIL.
Edition Bratislava, Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000, p. 85-85, 2000.
Publisher Slovenská chemická společnost
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Slovakia
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/00:00003324
Organization unit Faculty of Science
Keywords in English Capillary electrophoresis; Artychoke; Phenols
Tags Artychoke, Capillary electrophoresis, Phenols
Changed by Changed by: prof. RNDr. Zdeněk Glatz, CSc., učo 1865. Changed: 27/2/2001 11:01.
Abstract
Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative and hepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds present in the extract and to its caffeoylquinic derivatives. Micellar electrokinetic capillary chromatography (MECC) has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ion strength of the background electrolyte, SDS concentration and temperature. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic acid derivatives.
Links
GA303/00/D062, research and development projectName: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů
Investor: Czech Science Foundation, Bioavailability and mechanism of antioxidant activity of phenolic acids and lignans
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