Characterization of a fission yeast mutant which displays
defects in cell wall integrity and cytokinesis

D 2000

Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis

ISHIGURO, Junpei, Shin-ichiro SHIMADA, Miroslav GABRIEL and Marie KOPECKÁ

Basic information

Original name

Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis

Authors

ISHIGURO, Junpei (392 Japan, guarantor), Shin-ichiro SHIMADA (392 Japan), Miroslav GABRIEL (203 Czech Republic, belonging to the institution) and Marie KOPECKÁ (203 Czech Republic, belonging to the institution)

Edition

Delft University Press 2000. Delft University Press, The Netherlands, Tenth International Symposium on Yeasts The rising power of yeasts in science and industry, p. 173-173, 2 pp. 2000

Publisher

Johannes P. van Dijken and W.Alexander Scheffers (editors), Kluyver Lab. Biotechnol.Delft Universityx of Technology

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/00:00003325

Organization unit

Faculty of Medicine

ISBN

90-407-2067-3

UT WoS

000171979300007

Keywords in English

yeasts; fission; mutant; cell wall; cytokinesis

Abstract

V originále

Cps mutants of S.pombe were isolated as supersensitive to spindle poison isopropyl 3-N-chlorophenyl carbamate.Cps6-153 mutant reveals wall weakness and defects in cytokinesis and cell morphology. The aim of this work was to describe cytoskeleton and wall ultrastructure.Actin cytoskeleton reflected accumulation of wall material at poles and septum region.Cytoplasmic microtubules revealed abnormal alignment. The wall was less compact and more spongy with predominance of microfibrils.It is suggested that cps6 may function in regulation of cell wall integrity and cytokinesis in Ras-related signalling patway via interaction with the cytoskeletal organizaction.

Links

GA204/00/0394, research and development project

Name: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Czech Science Foundation, The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions

GA310/00/0391, research and development project

Name: Cytoskelet u lidských patogenních kvasinkových mikroorganismů.

Investor: Czech Science Foundation, Cytoskeleton in human pathogenic yeast microorganisms

Citovat

ISHIGURO, Junpei, Shin-ichiro SHIMADA, Miroslav GABRIEL and Marie KOPECKÁ. Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis. In Tenth International Symposium on Yeasts The rising power of yeasts in science and industry. Delft University Press 2000. Delft University Press, The Netherlands: Johannes P. van Dijken and W.Alexander Scheffers (editors), Kluyver Lab. Biotechnol.Delft Universityx of Technology, 2000, p. 173-173, 2 pp. ISBN 90-407-2067-3.

@inproceedings{331611,
   author = {Ishiguro, Junpei and Shimada, Shinandichiro and Gabriel, Miroslav and Kopecká, Marie},
   address = {Delft University Press, The Netherlands},
   booktitle = {Tenth International Symposium on Yeasts The rising power of yeasts in science and industry},
   edition = {Delft University Press 2000},
   keywords = {yeasts; fission; mutant; cell wall; cytokinesis},
   language = {eng},
   location = {Delft University Press, The Netherlands},
   isbn = {90-407-2067-3},
   pages = {173-173},
   publisher = {Johannes P. van Dijken and W.Alexander Scheffers (editors), Kluyver Lab. Biotechnol.Delft Universityx of Technology},
   title = {Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis},
   year = {2000}
}

TY  - JOUR
ID  - 331611
AU  - Ishiguro, Junpei - Shimada, Shin-ichiro - Gabriel, Miroslav - Kopecká, Marie
PY  - 2000
TI  - Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis
PB  - Johannes P. van Dijken and W.Alexander Scheffers (editors), Kluyver Lab. Biotechnol.Delft Universityx of Technology
CY  - Delft University Press, The Netherlands
SN  - 9040720673
KW  - yeasts
KW  - fission
KW  - mutant
KW  - cell wall
KW  - cytokinesis
N2  - Cps mutants of S.pombe were isolated as supersensitive to spindle poison isopropyl 3-N-chlorophenyl carbamate.Cps6-153 mutant reveals wall weakness and defects in cytokinesis and cell morphology. The aim of this work was to describe cytoskeleton and wall ultrastructure.Actin cytoskeleton reflected accumulation of wall material at poles and septum region.Cytoplasmic microtubules revealed abnormal alignment. The wall was less compact and more spongy with predominance of microfibrils.It is suggested that cps6 may function in regulation of cell wall integrity and cytokinesis in Ras-related signalling patway via interaction with the cytoskeletal organizaction.
ER  -

ISHIGURO, Junpei, Shin-ichiro SHIMADA, Miroslav GABRIEL and Marie KOPECKÁ. Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis. In \textit{Tenth International Symposium on Yeasts The rising power of yeasts in science and industry}. Delft University Press 2000. Delft University Press, The Netherlands: Johannes P. van Dijken and W.Alexander Scheffers (editors), Kluyver Lab. Biotechnol.Delft Universityx of Technology, 2000, p.~173-173, 2 pp. ISBN~90-407-2067-3.

Detailed Information on Publication Record