KAŠPAROVSKÝ, Tomáš, Lea LOJKOVÁ and Vladimír MIKEŠ. Mycelium extracts of Armillaria trigger a hypersensitive response in sensitive plant cells. In Book of Abstracts of EFPP Congres 2000, Taormina, Italy. Catania: EFPP, 2000, p. 63.
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Basic information
Original name Mycelium extracts of Armillaria trigger a hypersensitive response in sensitive plant cells
Authors KAŠPAROVSKÝ, Tomáš (203 Czech Republic, guarantor), Lea LOJKOVÁ and Vladimír MIKEŠ (203 Czech Republic).
Edition Catania, Book of Abstracts of EFPP Congres 2000, Taormina, Italy, p. 63-63, 2000.
Publisher EFPP
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Italy
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/00:00002368
Organization unit Faculty of Science
Keywords in English Elicitor; Armillaria; defence reactiion
Tags Armillaria, defence reactiion, elicitor
Changed by Changed by: prof. Mgr. Tomáš Kašparovský, Ph.D., učo 10078. Changed: 20/5/2009 16:43.
Abstract
The genus Armillaria includes serious pathogens causing root rot of a broad host range of plants. Water extracts of mycelium of A. tabescens inhibited the growth of Picea cell cultures and disturbed photosynthesis in leaves of Acer pseudoplatanus measured by fluorimetry. Extracts of mycelium of A. tabescens obtained by supercritical fluid extraction (SFE) trigger a hypersensitive response in suspension of tobacco cells accompanied by an alkalization of extracellular medium and production of active oxygen species. The HPLC analysis and GC-MS of SFE proved the presence of several toxins and sterols, namely ergosterol and dehydroergosterol. This reponse was calcium dependent and was stopped by inhibitors of protein kinases. At least two sterols had elicitor activity; one of them was ergosterol.
Abstract (in Czech)
The genus Armillaria includes serious pathogens causing root rot of a broad host range of plants. Water extracts of mycelium of A. tabescens inhibited the growth of Picea cell cultures and disturbed photosynthesis in leaves of Acer pseudoplatanus measured by fluorimetry. Extracts of mycelium of A. tabescens obtained by supercritical fluid extraction (SFE) trigger a hypersensitive response in suspension of tobacco cells accompanied by an alkalization of extracellular medium and production of active oxygen species. The HPLC analysis and GC-MS of SFE proved the presence of several toxins and sterols, namely ergosterol and dehydroergosterol. This reponse was calcium dependent and was stopped by inhibitors of protein kinases. At least two sterols had elicitor activity; one of them was ergosterol.
Links
MSM 143100005, plan (intention)Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism
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