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@article{339172,
author = {Říha, Karel and McKnight, Thomas and Fajkus, Jiří and Vyskot, Boris and Shippen, Dorothy E.},
article_location = {Velká Británie},
article_number = {5},
keywords = {telomere; telomerase; Silene latifolia; Arabidopsis thaliana; DNA replication},
language = {eng},
issn = {0960-7412},
journal = {The Plant Journal},
title = {Analysis of the G-overhang structures on plant telomeres: evidence for two distinct telomere architectures},
volume = {23},
year = {2000}
}
TY - JOUR
ID - 339172
AU - Říha, Karel - McKnight, Thomas - Fajkus, Jiří - Vyskot, Boris - Shippen, Dorothy E.
PY - 2000
TI - Analysis of the G-overhang structures on plant telomeres: evidence for two distinct telomere architectures
JF - The Plant Journal
VL - 23
IS - 5
SP - 633
EP - 633
PB - Blackwell Science Ltd
SN - 09607412
KW - telomere
KW - telomerase
KW - Silene latifolia
KW - Arabidopsis thaliana
KW - DNA replication
N2 - Telomeres are highly conserved structures essential for maintaining the integrity of eukaryotic genomes. In yeast, ciliates and mammals, the G-rich strand of the telomere forms a 3' overhang on the chromosome terminus. Here we investigate the architecture of telomeres in the dicot plants Silene latifolia and Arabidopsis thaliana using the PENT (primer extension/nick translation) assay. We show that both Arabidopsis and Silene telomeres carry G-overhangs longer than 20-30 nucleotides. However, in contrast to yeast and ciliate telomeres, only half of the telomeres in Silene seedlings possess detectable G-overhangs. PENT reactions using a variety of primers and reaction conditions revealed that the remaining fraction of Silene telomeres carries either no overhangs or overhangs less than 12 nucleotides in length. G-overhangs were observed in Silene seeds and leaves, tissues that lack telomerase activity. These findings suggest that incomplete DNA replication of the lagging strand, rather than synthesis by telomerase, is the primary mechanism for G-overhang synthesis in plants. Unexpectedly, we found that the fraction of telomeres with detectable G-overhangs decreased from 50% in seedlings to 35% in leaves. The difference may reflect increased susceptibility of the G-overhangs to nuclease attack in adult leaves, an event that could act as a precursor for the catabolic processes accompanying leaf senescence
ER -
ŘÍHA, Karel, Thomas MCKNIGHT, Jiří FAJKUS, Boris VYSKOT a Dorothy E. SHIPPEN. Analysis of the G-overhang structures on plant telomeres: evidence for two distinct telomere architectures. \textit{The Plant Journal}. Velká Británie: Blackwell Science Ltd, 2000, roč.~23, č.~5, s.~633-641. ISSN~0960-7412.
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