Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer

HOFR, Ctirad and Viktor BRABEC. Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2001, vol. 276, No 276, p. 9655-9661. ISSN 0021-9258.

Basic information

• Original name: Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer
• Authors: HOFR, Ctirad and Viktor BRABEC.
• Edition: Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2001, 0021-9258.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10610 Biophysics
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 7.258
• RIV identification code: RIV/00216224:14310/01:00003419
• Organization unit: Faculty of Science

Abstract

The effect of the site-specific interstrand cross-link formed by cisplatin or transplatin on the thermal stability and energetics of 20-base pairs DNA duplex is reported. The cross-linked or unplatinated 20-bp duplexes were investigated with the aid of differential scanning calorimetry, temperature-dependent UV absorption and circular dichroism. The cross-link of both platinum isomers increases the thermal stability of the modified duplexes by changing the molecularity of denaturation. The structural perturbation due to the interstrand cross-link of cisplatin increases entropy of the duplex. By contrast, the interstrand cross-link of transplatin is enthalpically almost neutral. These differences are consistent with distinct conformational distortions induced by the interstrand cross-links of the two isomers. It has been proposed that the results of the present work might be associated with distinctly different antitumor effects of these platinum compounds.

Links

• GA305/99/0695, research and development project: Name: Ovlivnění konformace DNA protinádorově účinnými komplexy kovů. Vztah k vývoji nových cytostatik.