Cytoskeleton and mitochondrial topology in Xantophyllomyces
dendrorhous (Phaffia rhodozyma)

J 2000

Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)

SLANINOVÁ, Iva, Judit KUCSERA and Augustin SVOBODA

Basic information

Original name

Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)

Authors

SLANINOVÁ, Iva, Judit KUCSERA and Augustin SVOBODA

Edition

Folia Microbiologica, Praha, MÚ ČAV, 2000, 0015-5632

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.752

RIV identification code

RIV/00216224:14110/00:00003447

Organization unit

Faculty of Medicine

Keywords in English

cytoskeleton; Xanthophyllomyces; Phaffia rhodozyma; microtubules; mitochondria

Abstract

V originále

The morphology of budding and conjugating cells and associated changes in microtubules and actin distribution were studied in the yeast Phaffia rhodozyma (Basidiomycetes) by phase-contrast and fluorescence microscopy. The non-budding interphase cell showed a nucleus situated in the central position and bundles of cytoplasmic microtubules either stretching parallel to the longitudinal cell axis or randomly distributed in the cell; none of these, however, were in contact with the nucleus. During mitosis, the nucleus divided in the daughter cell, cytoplasmic microtubules disappeared and were replaced by a spindle. The cytoplasmic microtubules reappeared after mitosis had finished. Actin patches were present in both the bud and the mother cell. Cells were induced to mate by nitrogen depletion. The partner cells fused at their conjugation projections where actin patches had been accumulated . Cell fusion resulted in a zygote that produced basidiophore with parallel bundles of microtubules extended along the basidiophore axis and with actin patches concentrated at the cell poles. The fused nucleus moved towards the basidiophore tip. During this movement, meiotic division was taking place; the nuclei were eventually distributed to basidiospores. Mitochondria visualised by means of DIOC6 (3) appeared as vesicles of various sizes; their large amounts were found, often lying adjacent to microtubules, in the cytoplasm of both vegetative cells and zygotes.

Links

GA204/00/0394, research and development project

Name: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Czech Science Foundation, The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions

GA204/99/D025, research and development project

Name: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Czech Science Foundation, The role of cytoskeleton and cell wall in the cell polarization

Citovat

SLANINOVÁ, Iva, Judit KUCSERA and Augustin SVOBODA. Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma). Folia Microbiologica. Praha: MÚ ČAV, 2000, vol. 45, No 1, p. 89. ISSN 0015-5632.

@article{344791,
   author = {Slaninová, Iva and Kucsera, Judit and Svoboda, Augustin},
   article_location = {Praha},
   article_number = {1},
   keywords = {cytoskeleton; Xanthophyllomyces; Phaffia rhodozyma; microtubules; mitochondria},
   language = {eng},
   issn = {0015-5632},
   journal = {Folia Microbiologica},
   title = {Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)},
   volume = {45},
   year = {2000}
}

TY  - JOUR
ID  - 344791
AU  - Slaninová, Iva - Kucsera, Judit - Svoboda, Augustin
PY  - 2000
TI  - Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)
JF  - Folia Microbiologica
VL  - 45
IS  - 1
SP  - 89
EP  - 89
PB  - MÚ ČAV
SN  - 00155632
KW  - cytoskeleton
KW  - Xanthophyllomyces
KW  - Phaffia rhodozyma
KW  - microtubules
KW  - mitochondria
N2  - The morphology of budding and conjugating cells and associated changes in microtubules and actin distribution were studied in the yeast Phaffia rhodozyma (Basidiomycetes) by phase-contrast and fluorescence microscopy. The non-budding interphase cell showed a nucleus situated in the central position and bundles of cytoplasmic microtubules either stretching parallel to the longitudinal cell axis or randomly distributed in the cell; none of these, however, were in contact with the nucleus. During mitosis, the nucleus divided in the daughter cell, cytoplasmic microtubules disappeared and were replaced by a spindle. The cytoplasmic microtubules reappeared after mitosis had finished. Actin patches were present in both the bud and the mother cell. Cells were induced to mate by nitrogen depletion. The partner cells fused at their conjugation projections where actin patches had been accumulated . Cell fusion resulted in a zygote that produced basidiophore with parallel bundles of microtubules extended along the basidiophore axis and with actin patches concentrated at the cell poles. The fused nucleus moved towards the basidiophore tip. During this movement, meiotic division was taking place; the nuclei were eventually distributed to basidiospores. Mitochondria visualised by means of DIOC6 (3) appeared as vesicles of various sizes; their large amounts were found, often lying adjacent to microtubules, in the cytoplasm of both vegetative cells and zygotes.
ER  -

SLANINOVÁ, Iva, Judit KUCSERA and Augustin SVOBODA. Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma). \textit{Folia Microbiologica}. Praha: MÚ ČAV, 2000, vol.~45, No~1, p.~89. ISSN~0015-5632.

Detailed Information on Publication Record