Detailed Information on Publication Record
2000
Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ and Jiří SLANINABasic information
Original name
Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky
Name (in English)
The effect of Chelidonius majus alkaloids on tumor and yeast cells
Authors
Edition
Cěské Budějovice, Cells II, p. 97-97, Cells II, 2000
Publisher
KOPP Publishing
Other information
Type of outcome
Stať ve sborníku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/00:00003448
Organization unit
Faculty of Medicine
Keywords in English
benzophenenthridine alkaloids; cytotoxicity; cytoskeleton;
Změněno: 20/12/2000 08:45, prof. MUDr. Iva Slaninová, Ph.D.
V originále
Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect at the concentration 1 mg/ml. In yeasts, neither microtubules nor cell growth were affected to any noticable extent even at the highest concentration (100 mg/ml) of the alkaloids.The dynamics of alkaloid distribution in cells was recorded by viewing fluorescence of alkaloids through filters with the excitation spectrum of 355 to 425 nm and 450 to 490 nm. In HeLa cells sanguinarine penetrated into nuclei few minutes after treatment while sanguilutine produced crystal-shaped bodies accumulated in the area of Golgi apparatus. Chelerythrine and chelilutine showed fluorescence in areas near the nucleus. Koptisine and berberine showed 3 type of fluorescence. Fluorescence of some lattice in cytoplasm, fluorescence of small vesicles and nucleoli fluorescence.
In English
Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect at the concentration 1 mg/ml. In yeasts, neither microtubules nor cell growth were affected to any noticable extent even at the highest concentration (100 mg/ml) of the alkaloids.The dynamics of alkaloid distribution in cells was recorded by viewing fluorescence of alkaloids through filters with the excitation spectrum of 355 to 425 nm and 450 to 490 nm. In HeLa cells sanguinarine penetrated into nuclei few minutes after treatment while sanguilutine produced crystal-shaped bodies accumulated in the area of Golgi apparatus. Chelerythrine and chelilutine showed fluorescence in areas near the nucleus. Koptisine and berberine showed 3 type of fluorescence. Fluorescence of some lattice in cytoplasm, fluorescence of small vesicles and nucleoli fluorescence.
Links
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