Migrating Schwann cells support the endoneurium of
freeze-treated nerve segment by laminins, fibronectin,
thrombospondin, and chondroitin sulfate.

D 2000

Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ

Basic information

Original name

Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.

Authors

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ

Edition

Praha, Sborník lékařský, p. 4-4, Vol. 101, No. 4, 2000

Publisher

Karlova Univerzita

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/00:00002596

Organization unit

Faculty of Medicine

Keywords in English

Schwann cell; extracellular matrix; peripheral nerve

Abstract

V originále

The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of the standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used a model of the freeze-treated nerve segment to study the molecular content of the endoneurium in absence and presence of Schwann cells. The sciatic nerves of 10 adult female rats were cut and the 10 mm long nerve segments were frozen 5 times with forceps pre-cooled in liquid nitrogen. The rats were secrificed after 14 days. Serial longitudinal frozen sections, 10 ľm thick, were incubated with antibodies against S-100 protein, laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The binding of primary antibodies was detected with indirect biotin-streptavidin-TRITC immunohistochemical method. Our results suggest that Schwann cells are crucial for axon-promoting condition in the endoneurium of the acellular nerve segment.

Links

GA309/00/0407, research and development project

Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves

GA309/97/0107, research and development project

Name: Exprese integrinů při diferenciaci Schwannových buněk a odvozených struktur v periferní nervové soustavě laboratorního potkana

Investor: Czech Science Foundation, Expression of integrins during differentiation of Schwann cells and derivative structures of the peripheral nervous system of the rat

Citovat

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ. Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate. In Sborník lékařský. Praha: Karlova Univerzita, 2000, p. 4. Vol. 101, No. 4.

@inproceedings{344836,
   author = {Dubový, Petr and Svíženská, Ivana and Klusáková, Ilona},
   address = {Praha},
   booktitle = {Sborník lékařský},
   keywords = {Schwann cell; extracellular matrix; peripheral nerve},
   language = {eng},
   location = {Praha},
   pages = {4-4},
   publisher = {Karlova Univerzita},
   title = {Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.},
   year = {2000}
}

TY  - JOUR
ID  - 344836
AU  - Dubový, Petr - Svíženská, Ivana - Klusáková, Ilona
PY  - 2000
TI  - Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate.
VL  - Vol. 101, No. 4
PB  - Karlova Univerzita
CY  - Praha
KW  - Schwann cell
KW  - extracellular matrix
KW  - peripheral nerve
N2  - The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of the standard acellular nerve segment in experimental application. It was believed that the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used a model of the freeze-treated nerve segment to study the molecular content of the endoneurium in absence and presence of Schwann cells. The sciatic nerves of 10 adult female rats were cut and the 10 mm long nerve segments were frozen 5 times with forceps pre-cooled in liquid nitrogen. The rats were secrificed after 14 days. Serial longitudinal frozen sections, 10 ľm thick, were incubated with antibodies against S-100 protein, laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The binding of primary antibodies was detected with indirect biotin-streptavidin-TRITC immunohistochemical method. Our results suggest that Schwann cells are crucial for axon-promoting condition in the endoneurium of the acellular nerve segment.
ER  -

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ and Ilona KLUSÁKOVÁ. Migrating Schwann cells support the endoneurium of freeze-treated nerve segment by laminins, fibronectin, thrombospondin, and chondroitin sulfate. In \textit{Sborník lékařský}. Praha: Karlova Univerzita, 2000, p.~4. Vol. 101, No. 4.

Detailed Information on Publication Record