Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique during in situ bioremediation

DAMBORSKÝ, Jiří, Martina DAMBORSKÁ, Svatopluk ŠTÍPEK, Andrea JESENSKÁ, Lukáš TRANTÍREK and Vladimír SKLENÁŘ. Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique during in situ bioremediation. Folia Microbiologica. vol. 45, No 1, p. 35-40. ISSN 0015-5632. 2000.

Basic information

• Original name: Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique during in situ bioremediation
• Authors: DAMBORSKÝ, Jiří (203 Czech Republic, guarantor), Martina DAMBORSKÁ, Svatopluk ŠTÍPEK, Andrea JESENSKÁ (203 Czech Republic), Lukáš TRANTÍREK (203 Czech Republic) and Vladimír SKLENÁŘ (203 Czech Republic).
• Edition: Folia Microbiologica, 2000, 0015-5632.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 0.752
• RIV identification code: RIV/00216224:14310/00:00002683
• Organization unit: Faculty of Science
• UT WoS: 000089765600004

Abstract

Spread-plating belongs to traditional microbiological methods employed for quantification of subsurface microilora during bioremediation projects in the Czechia. Concentration of degrading organisms is estimated from the number of colonies grown on agar p lates supplied with contaminant as the sole carbon source. The data obtained during in situ bioremediation of the Dacice site contaminated by cutting oil suggests that changes in the composition of the carbon source in the subsurface may cause a discrepancy between laboratory data and situation in subsurface.

Links

• GA203/97/P149, research and development project: Name: Studium molekulárních mechanismů biodegradačních reakcí - konstrukce QSBR modelů a proteinové inženýrství dehalogenas

Investor: Czech Science Foundation, Study of the molecular mechanisms of biodegradation reactions - construction of QSBR models and protein engineering of haloalkane dehalogenases

• ME 276, research and development project: Name: Racionální re-design mikrobiálních enzymů podílejících se na degradaci toxických organických polutantů

Investor: Ministry of Education, Youth and Sports of the CR, Rational re-design of microbial enzymes involved in degradation of toxic organic pollutants.

• MSM 143100005, plan (intention): Name: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular Structure-function Relationships and their role in the Metabolism