| VÉVODOVÁ, Jitka, Jaromír MAREK, Jan ZOUHAR, Břetislav BRZOBOHATÝ a X.-D. SU. Crystal structure of a cytokinic-glucoside-specific beta-glucosidase from Zea mays. Online. Biologické listy. Praha: Ústav molekulární genetiky AV ČR, 2000, roč. 65, 3-4, s. 250-252. ISSN 0366-0486. [citováno 2024-04-23] |
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@article{346186,
author = {Vévodová, Jitka and Marek, Jaromír and Zouhar, Jan and Brzobohatý, Břetislav and Su, X.andD.},
article_location = {Praha},
article_number = {3-4},
keywords = {crystal structure},
language = {eng},
issn = {0366-0486},
journal = {Biologické listy},
title = {Crystal structure of a cytokinic-glucoside-specific beta-glucosidase from Zea mays},
volume = {65},
year = {2000}
}
TY - JOUR
ID - 346186
AU - Vévodová, Jitka - Marek, Jaromír - Zouhar, Jan - Brzobohatý, Břetislav - Su, X.-D.
PY - 2000
TI - Crystal structure of a cytokinic-glucoside-specific beta-glucosidase from Zea mays
JF - Biologické listy
VL - 65
IS - 3-4
SP - 250
EP - 250
PB - Ústav molekulární genetiky AV ČR
SN - 03660486
KW - crystal structure
N2 - Zm-p60.1, a cytokinin-glucoside-specific b-glucosidase from maize, is a key enzyme involved in plant development and growth. It has been over-expressed in soluble form from Escherichia coli with a His tag at its N-terminus, the recombinant protein has been purified and crystallized at room temperature using PEG 4000 as main precipitant. A flash-annealed monoclinic crystal diffracted to high resolution (beyond 2 A) with space group P21, and unit-cell parameters a = 55.66 A, b = 110.72 A, c = 72.94 A, b = 92.10 deg. Resulting crystal structure was solved by molecular replacement.
ER -
VÉVODOVÁ, Jitka, Jaromír MAREK, Jan ZOUHAR, Břetislav BRZOBOHATÝ a X.-D. SU. Crystal structure of a cytokinic-glucoside-specific beta-glucosidase from Zea mays. Online. \textit{Biologické listy}. Praha: Ústav molekulární genetiky AV ČR, 2000, roč.~65, 3-4, s.~250-252. ISSN~0366-0486. [citováno 2024-04-23]
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