J 2000

Spatial arrangement of genes, centromeres and chromosomes in human blood cell nuclei and its changes during the cell cycle, differentiation and after irradiation

SKALNÍKOVÁ, Magdalena, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Pavla JIRSOVÁ et. al.

Basic information

Original name

Spatial arrangement of genes, centromeres and chromosomes in human blood cell nuclei and its changes during the cell cycle, differentiation and after irradiation

Authors

SKALNÍKOVÁ, Magdalena (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Alena CAFOURKOVÁ (203 Czech Republic), Irena KOUTNÁ (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic)

Edition

Chromosome Research, Dordrecht, Kluwer Academic, 2000, 0967-3849

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.725

RIV identification code

RIV/00216224:14330/00:00002779

Organization unit

Faculty of Informatics

UT WoS

000089606700004

Keywords in English

cell cycle; chromosomes; differentiation; image analysis; nuclear structure

Tags

Tags

International impact, Reviewed
Změněno: 7/5/2010 17:06, prof. RNDr. Michal Kozubek, Ph.D.

Abstract

V originále

Higher-order compartments of nuclear chromatin have been defined according to the replication timing, transcriptional activity, and information content (Ferreira et al. 1997, Sadoni et al. 1999). The results presented in this work contribute to this model of nuclear organization. Using different human blood cells, nuclear positioning of genes, centromeres, and whole chromosomes was investigated. Genes are located mostly in the interior of cell nuclei; centromeres are located near the nuclear periphery in agreement with the definition of the higher-order compartments. Genetic loci are found in specific subregions of cell nuclei which form distinct layers at defined centre-of-nucleus to locus distances. Inside these layers, the genetic loci are distributed randomly. Some chromosomes are polarized with genes located in the inner parts of the nucleus and centromere located on the nuclear periphery; polar organization was not found for some other chromosomes.

Links

IBS5004010, research and development project
Name: Vývoj nových diagnostických technik pro onkologii
Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
MSM 143300002, plan (intention)
Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
NM15, research and development project
Name: Užití interfázní FISH techniky pro vyhledávání lidí se zvýšeným rizikem vzniku zhoubných hemoblastóz
Investor: Ministry of Health of the CR, The use interphase FISH for the determination of individuals with elevated risk of the induction of hemoblastoses
VS97031, research and development project
Name: Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka
Investor: Ministry of Education, Youth and Sports of the CR, Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases