J 2000

An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy

KOZUBEK, Michal and Petr MATULA

Basic information

Original name

An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy

Authors

KOZUBEK, Michal (203 Czech Republic, guarantor, belonging to the institution) and Petr MATULA (203 Czech Republic, belonging to the institution)

Edition

Journal of Microscopy, Oxford, Blackwell Science, 2000, 0022-2720

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.173

RIV identification code

RIV/00216224:14330/00:00002780

Organization unit

Faculty of Informatics

UT WoS

000165550500004

Keywords in English

chromatic aberration; fluorescence microscopy; fluorescent bead; image analysis

Tags

Tags

International impact, Reviewed
Změněno: 29/1/2021 18:40, prof. RNDr. Michal Kozubek, Ph.D.

Abstract

V originále

Even the best optical microscopes available on the market exhibit chromatic aberrations to some extent. In some types of study, chromatic aberrations of current optics cannot be neglected and a software correction is highly desirable. This paper describes a novel method of chromatic aberration measurement and software correction using sub-resolution bead imaging and computer image analysis. The method is quick, precise and enables the determination of both longitudinal and lateral chromatic aberrations. Correction function can be computed in about half an hour, including image acquisition. Using this approach, chromatic aberrations can be reduced to 10-20 nm laterally and 10-60 nm axially depending on the type of optical set-up. The method is especially suitable for fluorescence microscopy, where a limited number of wavelengths are observed.

Links

GA202/99/P008, research and development project
Name: Využití analýzy obrazu při studiu struktury interfázního jádra
Investor: Czech Science Foundation, Image analysis in the study of interphase nucleus structure
IBS5004010, research and development project
Name: Vývoj nových diagnostických technik pro onkologii
Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
MSM 143300002, plan (intention)
Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
VS97031, research and development project
Name: Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka
Investor: Ministry of Education, Youth and Sports of the CR, Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases