KOZUBEK, Michal and Petr MATULA. An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy. Journal of Microscopy. Oxford: Blackwell Science, 2000, vol. 200, No 3, p. 206-217. ISSN 0022-2720.
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Basic information
Original name An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy
Authors KOZUBEK, Michal (203 Czech Republic, guarantor, belonging to the institution) and Petr MATULA (203 Czech Republic, belonging to the institution).
Edition Journal of Microscopy, Oxford, Blackwell Science, 2000, 0022-2720.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.173
RIV identification code RIV/00216224:14330/00:00002780
Organization unit Faculty of Informatics
UT WoS 000165550500004
Keywords in English chromatic aberration; fluorescence microscopy; fluorescent bead; image analysis
Tags cbia-web, chromatic aberration, fluorescence microscopy, fluorescent bead, Image analysis
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Michal Kozubek, Ph.D., učo 3740. Changed: 29/1/2021 18:40.
Abstract
Even the best optical microscopes available on the market exhibit chromatic aberrations to some extent. In some types of study, chromatic aberrations of current optics cannot be neglected and a software correction is highly desirable. This paper describes a novel method of chromatic aberration measurement and software correction using sub-resolution bead imaging and computer image analysis. The method is quick, precise and enables the determination of both longitudinal and lateral chromatic aberrations. Correction function can be computed in about half an hour, including image acquisition. Using this approach, chromatic aberrations can be reduced to 10-20 nm laterally and 10-60 nm axially depending on the type of optical set-up. The method is especially suitable for fluorescence microscopy, where a limited number of wavelengths are observed.
Links
GA202/99/P008, research and development projectName: Využití analýzy obrazu při studiu struktury interfázního jádra
Investor: Czech Science Foundation, Image analysis in the study of interphase nucleus structure
IBS5004010, research and development projectName: Vývoj nových diagnostických technik pro onkologii
Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
MSM 143300002, plan (intention)Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
VS97031, research and development projectName: Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka
Investor: Ministry of Education, Youth and Sports of the CR, Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases
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