Topography of genetic loci in tissue samples: towards new diagnostic tool using interphase FISH and high-resolution image analysis techniques

KOUTNÁ, Irena, Stanislav KOZUBEK, Jan ŽALOUDÍK, Michal KOZUBEK, Emilie LUKÁŠOVÁ, Pavel MATULA, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ, Alena CAFOURKOVÁ and Pavla JIRSOVÁ. Topography of genetic loci in tissue samples: towards new diagnostic tool using interphase FISH and high-resolution image analysis techniques. Analytical Cellular Pathology. Amsterdam: IOS Press, vol. 20, No 4, p. 173-185. ISSN 0921-8912. 2000.

Basic information

• Original name: Topography of genetic loci in tissue samples: towards new diagnostic tool using interphase FISH and high-resolution image analysis techniques
• Authors: KOUTNÁ, Irena (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Jan ŽALOUDÍK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Pavel MATULA (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Magdalena SKALNÍKOVÁ (203 Czech Republic), Alena CAFOURKOVÁ (203 Czech Republic) and Pavla JIRSOVÁ (203 Czech Republic).
• Edition: Analytical Cellular Pathology, Amsterdam, IOS Press, 2000, 0921-8912.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: abstract
• Impact factor: Impact factor: 0.838
• RIV identification code: RIV/00216224:14330/00:00002781
• Organization unit: Faculty of Informatics
• UT WoS: 000166980200004
• Keywords in English: FISH; high-resolution image analysis
• Tags: cbia-web
• Tags: International impact, Reviewed

Abstract

Using single and dual colour fluorescence in situ hybridisation (FISH) combined with image analysis techniques the topographic characteristics of genes and centromeres in nuclei of human colon tissue cells were investigated.

Links

• GA524/98/0190, research and development project: Name: Úloha endogenních antioxidantů v regulaci postischemického oxidativního stresu
• MSM 143300002, plan (intention): Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

• NC5955, research and development project: Name: Jak může přispět studium prostorového uspořádání specifických genetických lokusů v jádře buněk zdravých a maligních tkání k diagnostice a léčbě solidních tumorů.

Investor: Ministry of Health of the CR, How the study spatial order of specific genetic loci in nuclei of healthy and malignant tissues can contribute to the diagnostics and treatment of solid tumors.

• NM15, research and development project: Name: Užití interfázní FISH techniky pro vyhledávání lidí se zvýšeným rizikem vzniku zhoubných hemoblastóz

Investor: Ministry of Health of the CR, The use interphase FISH for the determination of individuals with elevated risk of the induction of hemoblastoses

• VS97031, research and development project: Name: Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka

Investor: Ministry of Education, Youth and Sports of the CR, Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases