GOLTSEV, Vasiliij, Todor Nikolaev GENKOV, Matej LEXA a Iordanka IVANOVA. Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro. Scientia Horticulturae, Amsterdam: Elsevier, 2001, roč. 89, č. 1, s. 41-55. ISSN 0304-4238.
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Základní údaje
Originální název Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro
Autoři GOLTSEV, Vasiliij, Todor Nikolaev GENKOV, Matej LEXA a Iordanka IVANOVA.
Vydání Scientia Horticulturae, Amsterdam, Elsevier, 2001, 0304-4238.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10610 Biophysics
Stát vydavatele Česko
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 0.510
Kód RIV RIV/00216224:14310/01:00004047
Organizační jednotka Přírodovědecká fakulta
UT WoS 000169276000004
Klíčová slova anglicky cytokinins; Dianthus caryophyllus; 4-PU-30; thidiazuron; photosystem II; chlorophyll fluorescence; delayed fluorescence
Štítky 4-PU-30, chlorophyll fluorescence, cytokinins, delayed fluorescence, Dianthus caryophyllus, photosystem II, thidiazuron
Změnil Změnil: Ing. Matej Lexa, Ph.D., učo 31298. Změněno: 26. 3. 2002 14:59.
Anotace
The effects of benzyladenine (BA) and two phenylurea cytokinins (N-phenyl-N'-(2-chloro-4-pyridyl)urea (4-PU-30) and thidiazuron (TDZ)) on in vitro cultures of carnation were studied using induction kinetics and thermograms of prompt and delayed chlorophyll fluorescence. In plantlets obtained from explants cultivated on a medium with 0.4 microM BA or 0.04-0.004 microM phenylurea cytokinins, the photosynthetic apparatus showed high PS II photochemical activity comparable to control without cytokinins and 5.8-97.5% higher estimated photoinduced transmembrane proton gradient (low concentrations of 4-PU-30 are especially effective). At a concentration of 0.4 microM, when compared to BA treatment, phenylurea cytokinins induced 24-35% suppression of photochemical activity, 44-46% suppression of estimated transmembrane proton gradient and 12-17% suppression of estimated electron transport rate. It is likely that this concentration of phenylurea cytokinins exceeded the optimal dose. The analysis of the same luminescent characteristics in the presence of different 4-PU-30 and TDZ concentrations showed that these compounds induced dose-dependent changes in the functional activity as well as alteration in the level of energization of the thylakoid membrane. When fluorescence thermograms were evaluated, phenylureas appeared to change the high temperature stability of the photosynthetic apparatus.
Návaznosti
MSM 143100008, záměrNázev: Genomy a jejich funkce
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumné záměry
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