GOLTSEV, Vasiliij, Todor Nikolaev GENKOV, Matej LEXA and Iordanka IVANOVA. Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro. Scientia Horticulturae. Amsterdam: Elsevier, 2001, vol. 89, No 1, p. 41-55. ISSN 0304-4238.
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Basic information
Original name Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro
Authors GOLTSEV, Vasiliij, Todor Nikolaev GENKOV, Matej LEXA and Iordanka IVANOVA.
Edition Scientia Horticulturae, Amsterdam, Elsevier, 2001, 0304-4238.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10610 Biophysics
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.510
RIV identification code RIV/00216224:14310/01:00004047
Organization unit Faculty of Science
UT WoS 000169276000004
Keywords in English cytokinins; Dianthus caryophyllus; 4-PU-30; thidiazuron; photosystem II; chlorophyll fluorescence; delayed fluorescence
Tags 4-PU-30, chlorophyll fluorescence, cytokinins, delayed fluorescence, Dianthus caryophyllus, photosystem II, thidiazuron
Changed by Changed by: doc. Ing. Matej Lexa, Ph.D., učo 31298. Changed: 26/3/2002 14:59.
Abstract
The effects of benzyladenine (BA) and two phenylurea cytokinins (N-phenyl-N'-(2-chloro-4-pyridyl)urea (4-PU-30) and thidiazuron (TDZ)) on in vitro cultures of carnation were studied using induction kinetics and thermograms of prompt and delayed chlorophyll fluorescence. In plantlets obtained from explants cultivated on a medium with 0.4 microM BA or 0.04-0.004 microM phenylurea cytokinins, the photosynthetic apparatus showed high PS II photochemical activity comparable to control without cytokinins and 5.8-97.5% higher estimated photoinduced transmembrane proton gradient (low concentrations of 4-PU-30 are especially effective). At a concentration of 0.4 microM, when compared to BA treatment, phenylurea cytokinins induced 24-35% suppression of photochemical activity, 44-46% suppression of estimated transmembrane proton gradient and 12-17% suppression of estimated electron transport rate. It is likely that this concentration of phenylurea cytokinins exceeded the optimal dose. The analysis of the same luminescent characteristics in the presence of different 4-PU-30 and TDZ concentrations showed that these compounds induced dose-dependent changes in the functional activity as well as alteration in the level of energization of the thylakoid membrane. When fluorescence thermograms were evaluated, phenylureas appeared to change the high temperature stability of the photosynthetic apparatus.
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MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
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