Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries

KLEPÁRNÍK, Karel, Zdenka MALÁ, Lubomír PŘIBYLA, Michaela BLAŽKOVÁ, Anna VAŠKŮ and Petr BOČEK. Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries. Electrophoresis. Weinheim: WILEY-VCH Verlag GmbH, 2000, vol. 21, No 21, p. 238-246. ISSN 0173-0835.

Basic information

• Original name: Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries
• Authors: KLEPÁRNÍK, Karel (203 Czech Republic, guarantor), Zdenka MALÁ (203 Czech Republic), Lubomír PŘIBYLA, Michaela BLAŽKOVÁ, Anna VAŠKŮ (203 Czech Republic) and Petr BOČEK.
• Edition: Electrophoresis, Weinheim, WILEY-VCH Verlag GmbH, 2000, 0173-0835.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.385
• RIV identification code: RIV/00216224:14110/00:00002891
• Organization unit: Faculty of Medicine
• UT WoS: 000085065800028
• Keywords in English: Denaturing capillary electrophoresis; microsatellite repeats; endothelin-1
• Tags: Denaturing capillary electrophoresis, Endothelin-1, microsatellite repeats

Abstract

The methodology and instrumentation for fast denaturing electrophoresis in short capillaries was developed and exemplified by detection of short tandem repeat (CA/CT) polymorphism in the endothelin-1 gene. The use of denaturing electrophoresis in short capillaries with laser-induced fluorescence detection resulted in a reduction of analysis time by a factor 200 when compared to the conventional slab gel electrophoresis.

Links

• GA306/96/0099, research and development project: Name: Polymorfismus genu pro endotelin - 1 (EDN 1) u esenciální hypertense

Investor: Czech Science Foundation, Polymorphism of endothelin - 1 gene (EDN 1) in essential hypertension

• MSM 141100002, plan (intention): Name: Molekulární patofyziologie multigenních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Molecular pathophysiology of multigene diseases

• VS96097, research and development project: Name: Molekulární patofyziologie vybraných "civilizačních", multigenně podmíněných chorob

Investor: Ministry of Education, Youth and Sports of the CR, Molecular pathophysiology of selected multigenic related to civilization diseases