Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe

GABRIEL, Miroslav, Junpei ISHIGURO and Marie KOPECKÁ. Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe (aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe). In VII. Cytoskeletální klub. 1st ed. Praha: Ústav molekulární genetiky Akademie věd, 2000, p. 11-11. VIII. Cytoskeletální klub.

Basic information

• Original name: Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe
• Authors: GABRIEL, Miroslav, Junpei ISHIGURO and Marie KOPECKÁ.
• Edition: 1. vyd. Praha, VII. Cytoskeletální klub, p. 11-11, 1 pp. VIII. Cytoskeletální klub, 2000.
• Publisher: Ústav molekulární genetiky Akademie věd

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/00:00003572
• Organization unit: Faculty of Medicine
• Keywords in English: fission yeast; cytoskeleton; ultrastructure; cps mutant
• Tags: cps mutant, Cytoskeleton, fission yeast, ultrastructure

Abstract

Cps6 mutant of the fission yeast is defective in cytokinesis, resulting in the formation of multiseptated cells.It is also more sensitive to digesting reagents than the wild type cells, suggesting that the mutant wall is altered in its composition or organization. The aim of this work was to examine wall and septum organization at the ultrastructural level and reinvestigate cytoskeleton in relation to aberrant phenotype.The results showed that the wall was less compact and more spongy with predominance of microfibrillar component.Lateral wall and septa were thicker. Septa remained unseparated.Cytoskeleton was well developed. Cps6 mutation may interfere with the termination of the synthesis of lateral wall and septum. Gene rad25+ coding for one of 14--3-3 homologs that complements cps6 mutation may be involved in pathway of cell wall integrity and proliferation.

Links

• GA204/00/0394, research and development project: Name: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Czech Science Foundation, The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions

• GA310/00/0391, research and development project: Name: Cytoskelet u lidských patogenních kvasinkových mikroorganismů.

Investor: Czech Science Foundation, Cytoskeleton in human pathogenic yeast microorganisms