Reinnervation of the rat biceps brachii muscle through the cellular and acellular nerve grafts introduced into the ventral horn of the spinal cord

HANINEC, Pavel, Petr DUBOVÝ, Ladislav HOUŠŤAVA and Ladislav STEJSKAL. Reinnervation of the rat biceps brachii muscle through the cellular and acellular nerve grafts introduced into the ventral horn of the spinal cord. In The AANS-CNS Book. 2000th ed. California: AANS-CNS, 2000, p. 101.

Basic information

• Original name: Reinnervation of the rat biceps brachii muscle through the cellular and acellular nerve grafts introduced into the ventral horn of the spinal cord
• Name in Czech: Reinervace m. biceps brachii přes celulární a acelulární nervový štěp
• Authors: HANINEC, Pavel (203 Czech Republic), Petr DUBOVÝ (203 Czech Republic, guarantor), Ladislav HOUŠŤAVA (203 Czech Republic) and Ladislav STEJSKAL (203 Czech Republic).
• Edition: 2000. vyd. California, The AANS-CNS Book, p. 101-101, 2000.
• Publisher: AANS-CNS

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/00:00003129
• Organization unit: Faculty of Medicine
• Keywords in English: acellular; cellular; nerve graft
• Tags: acellular, cellular, nerve graft

Abstract

The cellular and acellular nerve grafts were utilized to repair a functional connection between the motoneuron pool of the cervical spinal cord and the biceps brachii muscle through the musculocutaneous nerve. The were sutured with either cellular and acellular nerve grafts prepared from the saphenous nerve of the same animal. The one end of graft was sutured with the musculocutaneous nerve stump, the opposite end of the graft was introduced into the cervical spinal cord cranially to the avulsed C5 ventral root. The functional reinnervation of the biceps brachii muscle was evaluated by means of the behavioral (grooming) test, and electrophysiological methods. The presence of myelinated and unmyelinated axons in the nerve grafts and the musculocutaneous nerve stump was demonstrated by light and electron microscope. Our results demonstrate reinnervation of the musculocutaneous nerve via both cellular and acellular nerve grafts by axonal sprouts of the cervical motoneurons. Prior to axon regeneration, the acellular nerve graft was repopulated by Schwann cells migrating from the musculocutaneous nerve stump. The reinnervation by the way of acellular nerve graft was delayed and worse compared with the cellular graft.

Abstract (in Czech)

Celulární a acelulární nervový štěp byl použit k reinervaci m. biceps brachii.

Links

• GA309/00/0407, research and development project: Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves