| KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Hana ŠTĚRBOVÁ, Vladislav KAHLE, Jiří SLANINA a Pavel MUSIL. Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis. Journal of Chromatography A. Netherlands: Elsevier, 2001, roč. 916, (1+2), s. 265-271. ISSN 0021-9606. |
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@article{359471,
author = {Kvasničková, Leona and Glatz, Zdeněk and Štěrbová, Hana and Kahle, Vladislav and Slanina, Jiří and Musil, Pavel},
article_location = {Netherlands},
article_number = {(1+2)},
keywords = {Capillary electrochromatography; Polymer columns; Lignans; Schizandra},
language = {eng},
issn = {0021-9606},
journal = {Journal of Chromatography A},
title = {Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis},
volume = {916},
year = {2001}
}
TY - JOUR
ID - 359471
AU - Kvasničková, Leona - Glatz, Zdeněk - Štěrbová, Hana - Kahle, Vladislav - Slanina, Jiří - Musil, Pavel
PY - 2001
TI - Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis
JF - Journal of Chromatography A
VL - 916
IS - (1+2)
SP - 265
EP - 265
PB - Elsevier
SN - 00219606
KW - Capillary electrochromatography
KW - Polymer columns
KW - Lignans
KW - Schizandra
N2 - Capillary electrochromatography using polymer based monolithic stationary phase has been developed as a promising method for the determination of lignans of Schisandra chinensis. The columns were prepared by in situ copolymerisation of acrylamide, N,N-methylene-bis-acrylamide, vinylsulfonic acid and lauryl acrylate in presence of poly(ethylene glycol) as a porogenic agent. The columns (75 ěm inner diameter; 33 cm total length; 24.5 cm effective length) were successfully used to analyse and quantify the lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 35 min. The calibration graphs were linear in the range 0.025 - 1.0 mg/ml of given lignan with correlation coefficients between 0.9951 and 0.9996. The inter-day reproducibility of the peak area were below 3.9 % and the inter-day reproducibility of the migration time were below 4.2 %.The results of quantitative CEC analyses were compared with those obtained by reverse-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by HPLC.
ER -
KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Hana ŠTĚRBOVÁ, Vladislav KAHLE, Jiří SLANINA a Pavel MUSIL. Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis. \textit{Journal of Chromatography A}. Netherlands: Elsevier, 2001, roč.~916, (1+2), s.~265-271. ISSN~0021-9606.
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