Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea)
from five fish families (Teleostei)

J 2001

Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)

MATĚJUSOVÁ, Iveta, Milan GELNAR, Alastair J. A. MCBEATH, Catherine M. COLLINS, Carey O. CUNNINGHAM et. al.

Basic information

Original name

Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)

Authors

MATĚJUSOVÁ, Iveta, Milan GELNAR, Alastair J. A. MCBEATH, Catherine M. COLLINS and Carey O. CUNNINGHAM

Edition

International Journal for Parasitology, Australie, Elsevier Science, 2001, 0020-7519

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Australia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.814

RIV identification code

RIV/00216224:14310/01:00004169

Organization unit

Faculty of Science

Keywords in English

Gyrodactylus; rRNA; Polymerase chain reaction; Internal transcribed spacers; species-level identification

Abstract

V originále

Thirty-one gyrodactylid species from five families of freshwater fish were examined and variable region V4 of the 18S small subunit ribosomal RNA gene and ribosomal RNA internal transcribed spacers ITS1 and ITS2 were sequenced. Both the V4 region and spacers ITS1 and ITS2 proved useful for gyrodactylid diagnosis. Sequences of these fragments exhibited interspecific variations and allowed clear determination at the species level. In some cases, the length of the ITS1 PCR fragment provided useful genetic markers. Species that yielded a short ITS1 fragment also showed distinct groupings in ITS2 and V4 sequences that were markedly different to sequences from species that contain a long ITS1. Repetitive sequences located in the ITS1 of Gyrodactylus gobii and Gyrodactylus vimbi accounted for some of the variations in length of PCR products. There was no evidence for intraspecific variation within these regions and short tandem repeats were not found in the other species studied. The number of polymorphic and intraspecific variations in nucleic acid sequences was low, therefore these variations did not affect species determination of gyrodactylids. Minor differences in the sequenced between Western and Eastern European populations were detected for Gyrodactylus salaris/thymalli, Gyrodactylus teuchis and Gyrodactylus truttae, but these do not affect species diagnosis based on ribosomal DNA sequences. These results confirm the utility of both variable region V4 and the ITS as molecular markers for Gyrodactylus species.

Links

GA524/00/0844, research and development project

Name: Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

Investor: Czech Science Foundation, Neurobiology, surface architecture and microhabitat segregation of model parasite species during ontogenic development

GA524/98/0940, research and development project

Name: Diverzita parazitů jako indikátor enviromentálního stresu

Investor: Czech Science Foundation, Diversity of parasites like an indicator of environmental stress

MSM 143100010, plan (intention)

Name: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministry of Education, Youth and Sports of the CR, Spatiotemporal biodiversity dynamics in ecosystems of Central Europe

Citovat

MATĚJUSOVÁ, Iveta, Milan GELNAR, Alastair J. A. MCBEATH, Catherine M. COLLINS and Carey O. CUNNINGHAM. Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei). International Journal for Parasitology. Australie: Elsevier Science, 2001, vol. 31, No 4, p. 738-745. ISSN 0020-7519.

@article{361412,
   author = {Matějusová, Iveta and Gelnar, Milan and McBeath, Alastair J. A. and Collins, Catherine M. and Cunningham, Carey O.},
   article_location = {Australie},
   article_number = {4},
   keywords = {Gyrodactylus; rRNA; Polymerase chain reaction; Internal transcribed spacers; species-level identification},
   language = {eng},
   issn = {0020-7519},
   journal = {International Journal for Parasitology},
   title = {Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)},
   volume = {31},
   year = {2001}
}

TY  - JOUR
ID  - 361412
AU  - Matějusová, Iveta - Gelnar, Milan - McBeath, Alastair J. A. - Collins, Catherine M. - Cunningham, Carey O.
PY  - 2001
TI  - Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei)
JF  - International Journal for Parasitology
VL  - 31
IS  - 4
SP  - 738
EP  - 738
PB  - Elsevier Science
SN  - 00207519
KW  - Gyrodactylus
KW  - rRNA
KW  - Polymerase chain reaction
KW  - Internal transcribed spacers
KW  - species-level identification
N2  - Thirty-one gyrodactylid species from five families of freshwater fish were examined and variable region V4 of the 18S small subunit ribosomal RNA gene and ribosomal RNA internal transcribed spacers ITS1 and ITS2 were sequenced. Both the V4 region and spacers ITS1 and ITS2 proved useful for gyrodactylid diagnosis. Sequences of these fragments exhibited interspecific variations and allowed clear determination at the species level. In some cases, the length of the ITS1 PCR fragment provided useful genetic markers. Species that yielded a short ITS1 fragment also showed distinct groupings in ITS2 and V4 sequences that were markedly different to sequences from species that contain a long ITS1. Repetitive sequences located in the ITS1 of Gyrodactylus gobii and Gyrodactylus vimbi accounted for some of the variations in length of PCR products. There was no evidence for intraspecific variation within these regions and short tandem repeats were not found in the other species studied. The number of polymorphic and intraspecific variations in nucleic acid sequences was low, therefore these variations did not affect species determination of gyrodactylids. Minor differences in the sequenced between Western and Eastern European populations were detected for Gyrodactylus salaris/thymalli, Gyrodactylus teuchis and Gyrodactylus truttae, but these do not affect species diagnosis based on ribosomal DNA sequences. These results confirm the utility of both variable region V4 and the ITS as molecular markers for Gyrodactylus species.
ER  -

MATĚJUSOVÁ, Iveta, Milan GELNAR, Alastair J. A. MCBEATH, Catherine M. COLLINS and Carey O. CUNNINGHAM. Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei). \textit{International Journal for Parasitology}. Australie: Elsevier Science, 2001, vol.~31, No~4, p.~738-745. ISSN~0020-7519.

Detailed Information on Publication Record