ŽÍDEK, Lukáš, Petr DOLEŽEL, Josef CHMELÍK, Andrew G BAKER and Milos NOVOTNY. Modification of horse heart cytochrome c with trans-2-hexenal. Chemical Research in Toxicology. Washington, DC (USA): American Chemical Society, 1997, vol. 10, No 6, p. 702-710. ISSN 08993228X. |
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@article{362171, author = {Žídek, Lukáš and Doležel, Petr and Chmelík, Josef and Baker, Andrew G and Novotny, Milos}, article_location = {Washington, DC (USA)}, article_number = {6}, keywords = {ELECTROOSMOTIC ZONE DISPLACEMENT; LIPID-PEROXIDATION PRODUCTS; BOVINESERUM-ALBUMIN; PROTEIN ADDUCTS; ALDEHYDES}, issn = {08993228X}, journal = {Chemical Research in Toxicology}, title = {Modification of horse heart cytochrome c with trans-2-hexenal}, volume = {10}, year = {1997} }
TY - JOUR ID - 362171 AU - Žídek, Lukáš - Doležel, Petr - Chmelík, Josef - Baker, Andrew G - Novotny, Milos PY - 1997 TI - Modification of horse heart cytochrome c with trans-2-hexenal JF - Chemical Research in Toxicology VL - 10 IS - 6 SP - 702 EP - 702 PB - American Chemical Society SN - 08993228X KW - ELECTROOSMOTIC ZONE DISPLACEMENT KW - LIPID-PEROXIDATION PRODUCTS KW - BOVINESERUM-ALBUMIN KW - PROTEIN ADDUCTS KW - ALDEHYDES N2 - Horse heart cytochrome c reacting with trans-2-hexenal was used as a simple model of the nonspecific interactions of proteins with 2-alkenals. The reaction mixtures containing relatively high concentrations of the protein and aldehyde were characterized using visible spectrophotometry, fluorescence, and circular dichroism measurements, capillary isoelectric focusing, size-exclusion chromatography, polyacrylamide gel electrophoresis, and mass-spectrometric techniques. The mass-spectrometric data indicate that cytochrome c becomes modified with one or two molecules of hexenal as the major reaction product. The modified species with a correspondingly lowered isoelectric point were detected through capillary isoelectric focusing. The results of proteolytic studies indicate nonspecific modifications. Significant quantities of the oligomeric farms of hexenal-modified protein were also observed electrophoretically. ER -
ŽÍDEK, Lukáš, Petr DOLEŽEL, Josef CHMELÍK, Andrew G BAKER and Milos NOVOTNY. Modification of horse heart cytochrome c with trans-2-hexenal. \textit{Chemical Research in Toxicology}. Washington, DC (USA): American Chemical Society, 1997, vol.~10, No~6, p.~702-710. ISSN~08993228X.
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