| ŽÍDEK, Lukáš, Petr DOLEŽEL, Josef CHMELÍK, Andrew G BAKER and Milos NOVOTNY. Modification of horse heart cytochrome c with trans-2-hexenal. Chemical Research in Toxicology. Washington, DC (USA): American Chemical Society, 1997, vol. 10, No 6, p. 702-710. ISSN 08993228X. |
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@article{362171,
author = {Žídek, Lukáš and Doležel, Petr and Chmelík, Josef and Baker, Andrew G and Novotny, Milos},
article_location = {Washington, DC (USA)},
article_number = {6},
keywords = {ELECTROOSMOTIC ZONE DISPLACEMENT; LIPID-PEROXIDATION PRODUCTS; BOVINESERUM-ALBUMIN; PROTEIN ADDUCTS; ALDEHYDES},
issn = {08993228X},
journal = {Chemical Research in Toxicology},
title = {Modification of horse heart cytochrome c with trans-2-hexenal},
volume = {10},
year = {1997}
}
TY - JOUR
ID - 362171
AU - Žídek, Lukáš - Doležel, Petr - Chmelík, Josef - Baker, Andrew G - Novotny, Milos
PY - 1997
TI - Modification of horse heart cytochrome c with trans-2-hexenal
JF - Chemical Research in Toxicology
VL - 10
IS - 6
SP - 702
EP - 702
PB - American Chemical Society
SN - 08993228X
KW - ELECTROOSMOTIC ZONE DISPLACEMENT
KW - LIPID-PEROXIDATION PRODUCTS
KW - BOVINESERUM-ALBUMIN
KW - PROTEIN ADDUCTS
KW - ALDEHYDES
N2 - Horse heart cytochrome c reacting with trans-2-hexenal was used as a simple model of the nonspecific interactions of proteins with 2-alkenals. The reaction mixtures containing relatively high concentrations of the protein and aldehyde were characterized using visible spectrophotometry, fluorescence, and circular dichroism measurements, capillary isoelectric focusing, size-exclusion chromatography, polyacrylamide gel electrophoresis, and mass-spectrometric techniques. The mass-spectrometric data indicate that cytochrome c becomes modified with one or two molecules of hexenal as the major reaction product. The modified species with a correspondingly lowered isoelectric point were detected through capillary isoelectric focusing. The results of proteolytic studies indicate nonspecific modifications. Significant quantities of the oligomeric farms of hexenal-modified protein were also observed electrophoretically.
ER -
ŽÍDEK, Lukáš, Petr DOLEŽEL, Josef CHMELÍK, Andrew G BAKER and Milos NOVOTNY. Modification of horse heart cytochrome c with trans-2-hexenal. \textit{Chemical Research in Toxicology}. Washington, DC (USA): American Chemical Society, 1997, vol.~10, No~6, p.~702-710. ISSN~08993228X.
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