ŽÍDEK, Lukáš, Milos NOVOTNY and Martin J STONE. Increased protein backbone conformational entropy upon hydrophobic ligand binding. Nature Structural Biology. New York: Nature America Inc., 1999, vol. 6, No 12, p. 1118-1121. ISSN 1072-8368. |
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@article{362179, author = {Žídek, Lukáš and Novotny, Milos and Stone, Martin J}, article_location = {New York}, article_number = {12}, keywords = {N-15 NMR RELAXATION; NUCLEAR-MAGNETIC-RESONANCE; MODEL-FREE APPROACH; ORDER PARAMETERS; DYNAMICS; SPECTROSCOPY; RECOGNITION; ENERGY; DOMAIN; ENZYME}, issn = {1072-8368}, journal = {Nature Structural Biology}, title = {Increased protein backbone conformational entropy upon hydrophobic ligand binding}, volume = {6}, year = {1999} }
TY - JOUR ID - 362179 AU - Žídek, Lukáš - Novotny, Milos - Stone, Martin J PY - 1999 TI - Increased protein backbone conformational entropy upon hydrophobic ligand binding JF - Nature Structural Biology VL - 6 IS - 12 SP - 1118 EP - 1118 PB - Nature America Inc. SN - 10728368 KW - N-15 NMR RELAXATION KW - NUCLEAR-MAGNETIC-RESONANCE KW - MODEL-FREE APPROACH KW - ORDER PARAMETERS KW - DYNAMICS KW - SPECTROSCOPY KW - RECOGNITION KW - ENERGY KW - DOMAIN KW - ENZYME N2 - For complexes between proteins and very small hydrophobic ligands, hydrophobic effects alone map be insufficient to outweigh the unfavorable entropic terms resulting from bimolecular association. NMR relaxation experiments indicate that the backbone flexibility of mouse major urinary protein increases upon binding the hydrophobic mouse pheromone 2-sec-butyl-4,5-dihydrothiazole. The associated increase in backbone conformational entropy of the protein appears to make a substantial contribution toward stabilization of the protein-pheromone complex. This term is likely comparable in magnitude to other important free energy contributions to binding and map represent a general mechanism to promote binding of very small ligands to macromolecules. ER -
ŽÍDEK, Lukáš, Milos NOVOTNY and Martin J STONE. Increased protein backbone conformational entropy upon hydrophobic ligand binding. \textit{Nature Structural Biology}. New York: Nature America Inc., 1999, vol.~6, No~12, p.~1118-1121. ISSN~1072-8368.
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