Glycosylated major urinary protein of the house mouse: characterization of its N-linked oligosaccharides

MECHREF, Yehiya, Lukáš ŽÍDEK, Weidong MA and Milos NOVOTNY. Glycosylated major urinary protein of the house mouse: characterization of its N-linked oligosaccharides. Glycobiology. Oxford: Oxford University Press, 2000, vol. 10, No 3, p. 231-235. ISSN 0959-6658.

Basic information

Original name

Glycosylated major urinary protein of the house mouse: characterization of its N-linked oligosaccharides

Authors

MECHREF, Yehiya, Lukáš ŽÍDEK, Weidong MA and Milos NOVOTNY

Edition

Glycobiology, Oxford, Oxford University Press, 2000, 0959-6658

---

Other information

Type of outcome

Článek v odborném periodiku

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.419

Organization unit

Faculty of Science

Keywords in English

MASS-SPECTROMETRY; VOMERONASAL ORGAN; MUS-MUSCULUS; GENES; MATRIX; FAMILY; MICE

Tags

Family, GENES, MASS-SPECTROMETRY, matrix, mice, MUS-MUSCULUS, VOMERONASAL ORGAN

---

Abstract

V originále

A minor component of the major urinary protein complex of the house mouse was chromatographically isolated and ascertained to be a previously suspected glycoprotein. Using highly sensitive mass-spectrometric techniques for sequencing and linkage analysis, the N-linked oligosaccharides of this glycoprotein were characterized. They were determined to be of the complex type with a wide heterogeneity. The heterogeneity was due to both the degree of sialylation and the presence of galactose residues in either beta(1-3) or beta(1-4) linkages. The biantennary structures were the most pronounced glycans, while tri- and tetraantennary entities were minor.