Detailed Information on Publication Record
2001
Spatial distribution of selected genetic loci in nuclei of human leukemia cells after irradiation
JIRSOVÁ, Pavla, Stanislav KOZUBEK, Eva BÁRTOVÁ, Michal KOZUBEK, Emilie LUKÁŠOVÁ et. al.Basic information
Original name
Spatial distribution of selected genetic loci in nuclei of human leukemia cells after irradiation
Authors
JIRSOVÁ, Pavla (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Alena GAŇOVÁ (203 Czech Republic), Irena KOUTNÁ (203 Czech Republic) and Magdalena SKALNÍKOVÁ (203 Czech Republic)
Edition
Radiation Research, 2001, 0033-7587
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10610 Biophysics
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.478
RIV identification code
RIV/00216224:14330/01:00004205
Organization unit
Faculty of Informatics
UT WoS
000166708800006
Keywords in English
DOUBLE-STRAND BREAKS; SACCHAROMYCES-CEREVISIAE; HOMOLOGOUS RECOMBINATION; CHROMOSOME
Tags
International impact, Reviewed
Změněno: 7/5/2010 16:38, prof. RNDr. Michal Kozubek, Ph.D.
Abstract
V originále
Fluorescence in situ hybridization (FISH) combined with high-resolution cytometry was used to determine the topographic characteristics of the centromeric heterochromatin (of the chromosomes 6, 8, 9, 17) and the tumor suppressor gene TP53 (which is located on chromosome 17) in cells of the human leukemia cell lines ML-1 and U937. Analysis was performed on cells that were either untreated or irradiated with gamma rays and incubated for different intervals after exposure. Compared to untreated cells, homologous centromeres and the TP53 genes were found closer to each other and also closer to the nuclear center 2 h after irradiation. The spatial relationship between genetic elements returned to that of the unirradiated controls during the next 2-3 h. Statistical evaluation of our experimental results shows that homologous centromeres and the homologous genes are positioned closer to each other 2 h after irradiation because they are localized closer to the center of the nucleus (probably due to more pronounced decondensation of the chromatin related to repair), This radial movement of genetic loci, however, is not connected,vith repair of DSBs by processes involving homologous recombination, because the angular distribution of homologous sequences remains random after irradiation.
Links
| GA202/98/P253, research and development project |
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| MSM 143300002, plan (intention) |
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| NM15, research and development project |
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| VS97031, research and development project |
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