Exchange aberrations among 11 chromosomes of human lymphocytes induced by gamma-rays

GAŇOVÁ, Alena, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK, Michal KOZUBEK, Raisa GOVORUN, Irena KOUTNÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ, Pavla JIRSOVÁ, Renata PASEKOVÁ and Evgenij KRASAVIN. Exchange aberrations among 11 chromosomes of human lymphocytes induced by gamma-rays. International Journal of Radiation Biology. 2001, vol. 77, No 4, p. 419-429. ISSN 0955-3002.

Basic information

Original name

Exchange aberrations among 11 chromosomes of human lymphocytes induced by gamma-rays

Authors

GAŇOVÁ, Alena (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Raisa GOVORUN, Irena KOUTNÁ (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Magdalena SKALNÍKOVÁ (203 Czech Republic), Pavla JIRSOVÁ (203 Czech Republic), Renata PASEKOVÁ (203 Czech Republic) and Evgenij KRASAVIN

Edition

International Journal of Radiation Biology, 2001, 0955-3002

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

abstract

Impact factor

Impact factor: 2.356

RIV identification code

RIV/00216224:14330/01:00004206

Organization unit

Faculty of Informatics

UT WoS

000167998200003

Keywords in English

INDUCED STRUCTURAL-ABERRATIONS; INDUCED CHROMATIN BREAKS; IN-SITU HYBRIDIZATION; DOUBLE-STRAND BREAKS; IONIZING-RADIATION; CELL-NUCLEI; DOMAINS; TRANSLOCATIONS; REPAIR; ORGANIZATION

Tags

CELL-NUCLEI, DOMAINS, DOUBLE-STRAND BREAKS, IN-SITU HYBRIDIZATION, INDUCED CHROMATIN BREAKS, INDUCED STRUCTURAL-ABERRATIONS, IONIZING-RADIATION, organization, repair, TRANSLOCATIONS

Tags

International impact, Reviewed

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Abstract

V originále

Purpose: To detect the frequencies of inter-changes among 11 chromosomes in lymphocytes irradiated with gamma -rays and to find out whether these frequencies reflect the proximity of some of these chromosomes within the interphase nucleus. Material and methods: Exchange aberrations were detected in the first mitosis after irradiation of human lymphocytes with 3 and 5 Gy gamma -rays of` Co-60. Two-colour repeated FISH with two differently chemically modified probes in each hybridization was applied. The microscope stage positions of each mitosis were recorded after the first hybridization and used for the automatic scanning of images after all successive experiments. Five images were obtained for each mitosis differing in visualized pairs of chromosomes. Comparing these images, exchanges among 10 chromosomes could be detected. Painting of the p arm of chromosome 21 with the painting probe for chromosome 22 also made it possible to detect exchanges of this chromosome with other chromosomes of the selected group. Results: Frequencies of exchange aberrations induced in chromosomes of the selected group as well as interchanges between many pairs of chromosomes of this group were roughly proportional to the DNA content of chromosomes. Higher frequencies of interchanges than expected according to the model of linear proportionality were found between several chromosomes involved in translocations frequent in different subtypes of leukaemia. Conclusions: Frequencies of interchanges among 11 chromosomes of human lymphocytes induced by gamma -rays do not indicate as clearly as fast neutrons the non-random arrangement of chromosomes in the cell nucleus. The interaction of a large number of chromosomes in exchange aberrations suggests that the chromatirl in the territory of one chromosome is accessible for several other chromosomes.

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Links

GA202/01/0197, research and development project	Name: Ionizující záření jako nástroj pro výzkum struktury buněčného jádra a pro vývoj nových technik pro ekologii a medicínu Investor: Czech Science Foundation, Ionising radiation as a tool in the investigations of the cell nuclear structure and in the development of new techniques for ecology and medicine
GA524/98/0190, research and development project	Name: Úloha endogenních antioxidantů v regulaci postischemického oxidativního stresu
IBS5004010, research and development project	Name: Vývoj nových diagnostických technik pro onkologii Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology
MSM 143300002, plan (intention)	Name: Využití počítačové analýzy obrazu v optické mikroskopii Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
NC5955, research and development project	Name: Jak může přispět studium prostorového uspořádání specifických genetických lokusů v jádře buněk zdravých a maligních tkání k diagnostice a léčbě solidních tumorů. Investor: Ministry of Health of the CR, How the study spatial order of specific genetic loci in nuclei of healthy and malignant tissues can contribute to the diagnostics and treatment of solid tumors.
NM15, research and development project	Name: Užití interfázní FISH techniky pro vyhledávání lidí se zvýšeným rizikem vzniku zhoubných hemoblastóz Investor: Ministry of Health of the CR, The use interphase FISH for the determination of individuals with elevated risk of the induction of hemoblastoses