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@article{362475,
author = {Hofr, Ctirad and Farrell, Nicholas and Brabec, Viktor},
article_location = {Oxford, UK},
article_number = {10},
keywords = {DNA; thermodynamic stability; platinum drugs; calorimetry},
language = {eng},
issn = {0305-1048},
journal = {Nucleic Acids Research},
title = {Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex},
volume = {29},
year = {2001}
}
TY - JOUR
ID - 362475
AU - Hofr, Ctirad - Farrell, Nicholas - Brabec, Viktor
PY - 2001
TI - Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex
JF - Nucleic Acids Research
VL - 29
IS - 10
SP - 2034
EP - 2034
PB - Oxford Press
SN - 03051048
KW - DNA
KW - thermodynamic stability
KW - platinum drugs
KW - calorimetry
N2 - Bifunctional polynuclear platinum compounds represent a novel class of metal-based antitumor drugs. A typical agent is [{trans-PtCl(NH3)2}2H2N(CH2)4NH2]Cl2, (1,1/t,t) which coordinates to bases in DNA and forms covalent cross-links. It also forms a 1,2-d(GpG) intrastrand adduct, the equivalent of the major DNA lesion of "classical" cisplatin. In the present study, differential scanning calorimetry and spectroscopic techniques were employed to characterize the influence of this cross-link on the thermal stability and energetics of 20 bp DNA duplexes site-specifically modified by 1,1/t,t. Thermal denaturation data revealed that the cross-link of 1,1/t,t reduced thermal and thermodynamical stability of the duplex noticeably more than that of "classical" cisplatin. The energetic consequences of the intrastrand cross-link at the d(GG) site are discussed in relation to the structural distortions induced by this adduct in DNA and to its recognition and binding by HMG-domain proteins.
ER -
HOFR, Ctirad, Nicholas FARRELL a Viktor BRABEC. Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex. \textit{Nucleic Acids Research}. Oxford, UK: Oxford Press, roč.~29, č.~10, s.~2034-2040. ISSN~0305-1048. 2001.
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