J 2001

Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media

MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO, Viktor BRABEC et. al.

Basic information

Original name

Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media

Authors

MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO and Viktor BRABEC

Edition

Journal of Biological Inorganic Chemistry, germany, Springer-Verlag, 2001, 0949-368

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/01:00004215

Organization unit

Faculty of Science

Keywords in English

DNA; ruthenium; cytostatika; interakce
Změněno: 25/5/2001 15:15, prof. RNDr. Viktor Brabec, DrSc.

Abstract

V originále

Modifications of natural DNA by three anticancer heterocyclic ruthenium(III) compounds were studied by methods of molecular biophysics. These methods include: DNA binding studies using atomic absorption spectrophotometry, inhibition of restriction endonucleases, mapping of DNA adducts by transcription assay, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, circular dichroism analysis of B to Z transition in DNA and DNA melting curves measured by absorption spectrophotometry. It has been suggested that the altered DNA binding mode of ruthenium compounds in comparison with cisplatin might be an important factor responsible for altered cytostatic activity of this class of ruthenium compounds in tumor cells.

Links

GA305/99/0695, research and development project
Name: Ovlivnění konformace DNA protinádorově účinnými komplexy kovů. Vztah k vývoji nových cytostatik.