Detailed Information on Publication Record
2001
Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media
MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO, Viktor BRABEC et. al.Basic information
Original name
Biophysical analysis of natural, double-helical DNA modified by anticancer heterocyclic complexes of ruthenium(III) in cell-free media
Authors
MALINA, Jaroslav, Olga NOVÁKOVÁ, Bernhard K. KEPPLER, Enzo ALESSIO and Viktor BRABEC
Edition
Journal of Biological Inorganic Chemistry, germany, Springer-Verlag, 2001, 0949-368
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10610 Biophysics
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/01:00004215
Organization unit
Faculty of Science
Keywords in English
DNA; ruthenium; cytostatika; interakce
Tags
Změněno: 25/5/2001 15:15, prof. RNDr. Viktor Brabec, DrSc.
Abstract
V originále
Modifications of natural DNA by three anticancer heterocyclic ruthenium(III) compounds were studied by methods of molecular biophysics. These methods include: DNA binding studies using atomic absorption spectrophotometry, inhibition of restriction endonucleases, mapping of DNA adducts by transcription assay, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, circular dichroism analysis of B to Z transition in DNA and DNA melting curves measured by absorption spectrophotometry. It has been suggested that the altered DNA binding mode of ruthenium compounds in comparison with cisplatin might be an important factor responsible for altered cytostatic activity of this class of ruthenium compounds in tumor cells.
Links
GA305/99/0695, research and development project |
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