Detailed Information on Publication Record
2001
Determination of phenolic compounds in artichoke extract by means of MEKC
ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA and Pavel MUSILBasic information
Original name
Determination of phenolic compounds in artichoke extract by means of MEKC
Authors
ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA and Pavel MUSIL
Edition
1. vyd. Verona, Book of abstract ICES 2001 An Electrophoretic Odessey, p. 80-80, 2001
Publisher
Emmezeta Congressi
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Italy
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/01:00004276
Organization unit
Faculty of Science
Keywords in English
MEKC Schizandra Lignans
Změněno: 3/7/2001 11:51, prof. RNDr. Zdeněk Glatz, CSc.
Abstract
V originále
Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative and hepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds presented in the extract and to their caffeoylquinic derivatives. MEKC has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ionic strength of the background electrolyte, SDS concentration and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic acid derivatives.
Links
| GA303/00/D062, research and development project |
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