ZEHNULOVÁ, Jana, Jana KAŠPÁRKOVÁ, Nicholas FARRELL and Viktor BRABEC. Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol., 2001, vol. 276, No 25, p. 22191-22199. ISSN 0021-9258. |
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@article{365796, author = {Zehnulová, Jana and Kašpárková, Jana and Farrell, Nicholas and Brabec, Viktor}, article_location = {Bethesda, USA}, article_number = {25}, keywords = {DNA; anticancer drug; platinum; conformation; recognition}, language = {eng}, issn = {0021-9258}, journal = {Journal of Biological Chemistry}, title = {Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464}, volume = {276}, year = {2001} }
TY - JOUR ID - 365796 AU - Zehnulová, Jana - Kašpárková, Jana - Farrell, Nicholas - Brabec, Viktor PY - 2001 TI - Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464 JF - Journal of Biological Chemistry VL - 276 IS - 25 SP - 22191 EP - 22191 PB - Amer. Soc. Biochem. Mol. SN - 00219258 KW - DNA KW - anticancer drug KW - platinum KW - conformation KW - recognition N2 - The new antitumor trinuclear platinum compound [{trans-PtCl(NH3)2}2m- trans-Pt(NH3)2{H2N(CH2)6NH2}2]4+ (designated as BBR3464) is currently in Phase II clinical trials. DNA is generally considered the major pharmacological target of platinum drugs. The bifunctional DNA binding of BBR3464 is characterized by the rapid formation of long-range intra- and interstrand cross-links. We examined how the structures of the various types of the intrastrand cross-links of BBR3464 affect conformational properties of DNA, how these adducts are recognized by HMG1 protein and removed from DNA during in vitro nucleotide excision repair reactions. The results have revealed that intrastrand cross-links of BBR3464 create a local conformational distortion, but none of these cross-links results in a stable curvature. In addition, we have observed no recognition of these cross-links by HMG1 proteins, but we have observed effective removal of these adducts from DNA by nucleotide excision repair. ER -
ZEHNULOVÁ, Jana, Jana KAŠPÁRKOVÁ, Nicholas FARRELL and Viktor BRABEC. Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol., 2001, vol.~276, No~25, p.~22191-22199. ISSN~0021-9258.
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