Interaction of benzo[c]phenanthridine and protoberberine
alkaloids with animal and yeast cells

J 2001

Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells

SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ and Jiří SLANINA

Basic information

Original name

Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells

Authors

SLANINOVÁ, Iva (203 Czech Republic, guarantor), Eva TÁBORSKÁ (203 Czech Republic), Hana BOCHOŘÁKOVÁ (203 Czech Republic) and Jiří SLANINA (203 Czech Republic)

Edition

Cell Biology and Toxicology, Dordrecht, Netherlands, Kluwer Academic Publishers, 2001, 0742-2091

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.177

RIV identification code

RIV/00216224:14110/01:00004307

Organization unit

Faculty of Medicine

UT WoS

000169125300006

Keywords in English

benzo[c]phenanthridine; protoberberine alkaloids; cytotoxicity; yeast; cytoskeleton; microtubules

Abstract

V originále

We compared the effects of four quaternary benzo[c]phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine, and two quaternary protoberberine alkaloids, berberine and coptisine, on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis. The ability of alkaloids to display primary fluorescence, allowed us to record their dynamics and localization in cells. Cytotoxicity, anti-microtubular and anti-actin effects in living cells were studied. In the yeasts, neither microtubules nor cell growth were seriously affected even at the alkaloid concentration of 100 mg/mL. The HeLa cells, however, responded to the toxic effect of alkaloids at concentrations ranging from 1-50 mg/mL. IC50 values for individual alkaloids were: sanguinarine IC50 = 0.8 mg/mL, sanguilutine IC50 = 8.3 mg/mL, chelerythrine IC50 = 6.2 mg/mL, chelilutine IC50 = 5.2 mg/mL, coptisine IC50 = 2.6 mg/mL and berberine IC50 =10.0 mg/mL. In living cells, sanguinarine produced a decrease in microtubule numbers, particularly at the cell periphery, at a concentration of 0.1 mg/mL The other alkaloids show the similar effect but at higher concentrations (5 to 10 mg/mL). The strongest effects of sanguinarine were explained as a consequence of its easy penetration through the cell membrane due to non-polar pseudobase formation and to a high degree of molecular planarity.

Links

GA204/99/D025, research and development project

Name: Úloha cytoskeletu a buněčné stěny v polarizaci buňky

Investor: Czech Science Foundation, The role of cytoskeleton and cell wall in the cell polarization

Citovat

SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ and Jiří SLANINA. Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells. Cell Biology and Toxicology. Dordrecht, Netherlands: Kluwer Academic Publishers, 2001, vol. 17, No 1, p. 51-63. ISSN 0742-2091.

@article{365975,
   author = {Slaninová, Iva and Táborská, Eva and Bochořáková, Hana and Slanina, Jiří},
   article_location = {Dordrecht, Netherlands},
   article_number = {1},
   keywords = {benzo[c]phenanthridine; protoberberine alkaloids; cytotoxicity; yeast; cytoskeleton; microtubules},
   language = {eng},
   issn = {0742-2091},
   journal = {Cell Biology and Toxicology},
   title = {Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells},
   volume = {17},
   year = {2001}
}

TY  - JOUR
ID  - 365975
AU  - Slaninová, Iva - Táborská, Eva - Bochořáková, Hana - Slanina, Jiří
PY  - 2001
TI  - Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells
JF  - Cell Biology and Toxicology
VL  - 17
IS  - 1
SP  - 51
EP  - 51
PB  - Kluwer Academic Publishers
SN  - 07422091
KW  - benzo[c]phenanthridine
KW  - protoberberine alkaloids
KW  - cytotoxicity
KW  - yeast
KW  - cytoskeleton
KW  - microtubules
N2  - We compared the effects of four quaternary benzo[c]phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine, and two quaternary protoberberine alkaloids, berberine and coptisine, on the human cell line HeLa (cervix carcinoma cells) and the yeasts Saccharomyces cerevisiae and Schizosaccharomyces japonicus var. versatilis. The ability of alkaloids to display primary fluorescence, allowed us to record their dynamics and localization in cells. Cytotoxicity, anti-microtubular and anti-actin effects in living cells were studied. In the yeasts, neither microtubules nor cell growth were seriously affected even at the alkaloid concentration of 100 mg/mL. The HeLa cells, however, responded to the toxic effect of alkaloids at concentrations ranging from 1-50 mg/mL. IC50 values for individual alkaloids were: sanguinarine IC50 = 0.8 mg/mL, sanguilutine IC50 = 8.3 mg/mL, chelerythrine IC50 = 6.2 mg/mL, chelilutine IC50 = 5.2 mg/mL, coptisine IC50 = 2.6 mg/mL and berberine IC50 =10.0 mg/mL. In living cells, sanguinarine produced a decrease in microtubule numbers, particularly at the cell periphery, at a concentration of 0.1 mg/mL The other alkaloids show the similar effect but at higher concentrations (5 to 10 mg/mL). The strongest effects of sanguinarine were explained as a consequence of its easy penetration through the cell membrane due to non-polar pseudobase formation and to a high degree of molecular planarity.
ER  -

SLANINOVÁ, Iva, Eva TÁBORSKÁ, Hana BOCHOŘÁKOVÁ and Jiří SLANINA. Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells. \textit{Cell Biology and Toxicology}. Dordrecht, Netherlands: Kluwer Academic Publishers, 2001, vol.~17, No~1, p.~51-63. ISSN~0742-2091.

Detailed Information on Publication Record