Detailed Information on Publication Record
2001
Combined confocal and wide-field high-resolution cytometry of FISH-stained cells
KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ et. al.Basic information
Original name
Combined confocal and wide-field high-resolution cytometry of FISH-stained cells
Authors
KOZUBEK, Michal (203 Czech Republic, guarantor, belonging to the institution), Stanislav KOZUBEK (203 Czech Republic), Emilie LUKÁŠOVÁ (203 Czech Republic), Eva BÁRTOVÁ (203 Czech Republic), Magdalena SKALNÍKOVÁ (203 Czech Republic, belonging to the institution), Pavel MATULA (203 Czech Republic, belonging to the institution), Petr MATULA (203 Czech Republic, belonging to the institution), Pavla JIRSOVÁ (203 Czech Republic, belonging to the institution), Alena GAŇOVÁ (203 Czech Republic, belonging to the institution) and Irena KOUTNÁ (203 Czech Republic, belonging to the institution)
Edition
Cytometry, New York, International Society for Analytical Cyt, 2001, 0196-4763
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
20200 2.2 Electrical engineering, Electronic engineering, Information engineering
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.220
RIV identification code
RIV/00216224:14330/01:00004339
Organization unit
Faculty of Informatics
UT WoS
000171015000001
Keywords in English
high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; fluorescence microscopy; automated microscopy; image analysis; 3-D analysis
Tags
Tags
International impact, Reviewed
Změněno: 29/1/2021 18:38, prof. RNDr. Michal Kozubek, Ph.D.
Abstract
V originále
The recently developed technique of high-resolution cytometry (HRCM) enables automated acquisition and analysis of FISH stained cell nuclei using wide-field fluorescence microscopy. The method has now been extended to confocal imaging and offers the opportunity to combine the advantages of confocal and wide-field modes. Using the combined confocal and wide-field HRCM technique, it is possible to take advantage of both imaging modes. Images of some dyes (such as small hybridization dots or counterstain images of individual interphase nuclei) do not require confocal quality and can be acquired quickly in wide-field mode. On the contrary, images of other dyes (such as chromosome territories or counterstain images of cells in tissues) do require improved quality and are acquired in confocal mode. The dual-mode approach is 2-3 times faster compared with the single-mode confocal approach and the spectrum of its applications is much broader compared with both single-mode confocal and single-mode wide-field systems. The combination of high speed specific to the wide-field mode and high quality specific to the confocal mode gives optimal system performance.
Links
| GA202/99/P008, research and development project |
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| IBS5004010, research and development project |
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| MSM 143300002, plan (intention) |
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| VS97031, research and development project |
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