Detailed Information on Publication Record
2001
Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae)
MATĚJUSOVÁ, Iveta, Božena KOUBKOVÁ, Stefano D`AMELIO and Carey O. CUNNINGHAMBasic information
Original name
Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae)
Authors
MATĚJUSOVÁ, Iveta, Božena KOUBKOVÁ, Stefano D`AMELIO and Carey O. CUNNINGHAM
Edition
Parasitology, United Kingdom, Cambridge University Press, 2001, 0031-1820
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.114
RIV identification code
RIV/00216224:14310/01:00004340
Organization unit
Faculty of Science
Keywords in English
Monogenea; Diplozoidae; PCR; ITS2; RFLP; genetic distance
Tags
Změněno: 7/12/2001 10:57, Mgr. Iveta Matějusová, Ph.D.
Abstract
V originále
The second internal transcribed spacer (ITS2)of the ribosomal RNA gene array of 6 species of diplozoids was amplified by PCR and sequenced. These sequences clearly demonstrate discrimination at the species level and confirm the validity of species determined by morphological identification. No intraspecific variation was found in the ITS2 sequences. There were no differences in the ITS2 sequenced of P. homoion from populations parasitizing different host species. The length of the PCR product allowed discrimination of E. nipponicum from the Paradiplozoon species. Digestion of the amplified ITS2 fragment with enzymes AluI, HaeIII and HifI provided useful genetic markers for species identification. This represent the first molecular taxonomic study of these interesting parasites and demonstrates the utility of these methods for addressing questions of systematics.
Links
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