| MATĚJUSOVÁ, Iveta, Božena KOUBKOVÁ, Stefano D`AMELIO and Carey O. CUNNINGHAM. Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae). Parasitology. United Kingdom: Cambridge University Press, 2001, vol. 123, -, p. 465-474. ISSN 0031-1820. |
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@article{366413,
author = {Matějusová, Iveta and Koubková, Božena and D`Amelio, Stefano and Cunningham, Carey O.},
article_location = {United Kingdom},
article_number = {-},
keywords = {Monogenea; Diplozoidae; PCR; ITS2; RFLP; genetic distance},
language = {eng},
issn = {0031-1820},
journal = {Parasitology},
title = {Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae)},
volume = {123},
year = {2001}
}
TY - JOUR
ID - 366413
AU - Matějusová, Iveta - Koubková, Božena - D`Amelio, Stefano - Cunningham, Carey O.
PY - 2001
TI - Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae)
JF - Parasitology
VL - 123
IS - -
SP - 465
EP - 465
PB - Cambridge University Press
SN - 00311820
KW - Monogenea
KW - Diplozoidae
KW - PCR
KW - ITS2
KW - RFLP
KW - genetic distance
N2 - The second internal transcribed spacer (ITS2)of the ribosomal RNA gene array of 6 species of diplozoids was amplified by PCR and sequenced. These sequences clearly demonstrate discrimination at the species level and confirm the validity of species determined by morphological identification. No intraspecific variation was found in the ITS2 sequences. There were no differences in the ITS2 sequenced of P. homoion from populations parasitizing different host species. The length of the PCR product allowed discrimination of E. nipponicum from the Paradiplozoon species. Digestion of the amplified ITS2 fragment with enzymes AluI, HaeIII and HifI provided useful genetic markers for species identification. This represent the first molecular taxonomic study of these interesting parasites and demonstrates the utility of these methods for addressing questions of systematics.
ER -
MATĚJUSOVÁ, Iveta, Božena KOUBKOVÁ, Stefano D`AMELIO and Carey O. CUNNINGHAM. Genetic characterization of six species of diplozoids (Monogenea, Diplozoidae). \textit{Parasitology}. United Kingdom: Cambridge University Press, 2001, vol.~123, -, p.~465-474. ISSN~0031-1820.
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